This is for the Geneticists, Bio-Geeks, and the synthetic biology crowd here on Google Plus. Recently I proposed a method to determine what RNA or mRNA cloned cells use to extend their telomeres. I haven't gotten a any real response so I thought I would post the question here.
The thought is simple, every time a cell is cloned its telomeres must be getting longer. Something in the egg cell is causing the extension of the telomeres.That something must be either telomerase or one or more pieces of mRMA. The fact that we have been able to clone 25 successive generations of mice, and make pluripotent stem cells via cloning seems to point to telomere extension.
Recently a technique to PCR the mRNA in individual cells was developed.
Now the question How difficult would it be to determine which mRNA fragments in egg cells are causing the telomere extension?
Telomere - http://en.wikipedia.org/wiki/Telomere
Telomerase - http://en.wikipedia.org/wiki/Telomerase
Cloning 25 generations of mice - http://www.eurekalert.org/pub_releases/2013-03/r-jrs030613.php
Cloning Pluripotent Stem Cells - http://www.sciencenews.org/view/generic/id/350432/description/Cloning_produces_human_embryonic_stem_cells
PCR of a single cells mRNA - http://www.nanowerk.com/news2/biotech/newsid=31189.php