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Profiling Human Islet Cells with Laser Capture Microdissection
Laser capture microdissection successfully isolates cellular preparations for characterizing the islet cell proteome in Type I diabetes research.
https://www.thermofisher.com/blog/proteomics/profiling-human-islet-cells-with-laser-capture-microdissection/
Instrumentation includes an UltiMate 3000 ultra-high-performance LC system followed by a mass spectrometry–based label-free proteomics approach with nanoLC–mass spectrometry using a Q Exactive HF mass spectrometer.
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 Obese and Healthy? Proteomic Biomarkers Give Clues
Shotgun proteomics with label-free quantitation characterizes the serum proteome for biomarker evidence of metabolically healthy obesity, showing that harmful metabolic pathways are downregulated.
https://www.thermofisher.com/blog/proteomics/obese-and-healthy-proteomic-biomarkers-give-clues/
Instrumentation includes an LTQ ion trap mass spectrometer
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Autophagy Helps Control Endothelial Permeability
Mass spectrometric proteomic analysis of HUVEC cell preparations shows effect of autophagy on endothelial cell and vessel wall characteristics.
https://www.thermofisher.com/blog/proteomics/autophagy-helps-control-endothelial-permeability/
Instrumentation includes an EASY-nLC liquid chromatograph and an LTQ Orbitrap Elite mass spectrometer, with si-ATG5 RNA-Stealth, an Invitrogen Click-iT EdU flow cytometry assay kit and an Annexin V apoptosis kit used in the cell culture process.
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A Role for Decorin Peptides in Atrial Fibrillation
LC-MS/MS to compare atrial proteomes in normal sinus rhythm and following atrial fibrillation shows decorin protein damage in the cardiac ECM.
http://admin.acceleratingscience.com/proteomics/a-role-for-decorin-peptides-in-atrial-fibrillation/
Instrumentation includes an Orbitrap Elite mass spectrometer.
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Proteomic Changes in Glaucoma
Accurate inclusion mass screening and targeted LC-MS/MS proteomics identifies human retinal proteins affected by glaucoma.
https://www.thermofisher.com/blog/proteomics/proteomic-changes-in-glaucoma/
Instrumentation includes a 30 × 0.5 mm BioBasic C18 column coupled to a 150 × 0.5 mm BioBasic C18 column LC system with an LTQ Orbitrap XL Hybrid Ion Trap-Orbitrap mass spectrometer with a low flow metal needle, using Xcalibur software, revision 2.0.7 SP1 to process data.
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Investigating Nephrotoxicity from Herbal Medicines
HILIC-ESI-MS/MS workflow characterizes strychnine and brucine alkaloid abundance in addition to tyrosine and tyramine as markers of herbal therapy-induced damage in an HEK 293t cell culture model of S. Strychni nephrotoxicity.
https://www.thermofisher.com/blog/proteomics/investigating-nephrotoxicity-from-herbal-medicines/
Instrumentation includes a Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer .
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Crocodile Tears: The Metabolic Fate and Detection of Desomorphine
LC-HR-MS/MS workflows characterize desomorphine metabolites in rat urine, human liver microsomes and hepatic cell culture preparations to enable urinalysis for opioid screening in cases of toxicosis or abuse.

https://www.thermofisher.com/blog/proteomics/crocodile-tears-the-metabolic-fate-and-detection-of-desomorphine/
Instrumentation includes an Accela LC system coupled with a Q Exactive mass spectrometer controlled by Xcalibur software and hydrophilic interaction LC-HR-MS/MS, using an UltiMate 3000 rapid separation system and a Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer.
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Unique Proteome Signature in Post-chemotherapy Ovarian Cancer
Novel separation technique isolates tumor cells from ascetic fluid collected pre- and post-chemotherapy to characterize protein changes associated with chemoresistance and recurrence.
https://www.thermofisher.com/blog/proteomics/unique-proteome-signature-in-post-chemotherapy-ovarian-cancer/
Instrumentation includes an UltiMate 3000 RSLCnano System ultra-performance liquid chromatography instrument coupled online to an Orbitrap Elite mass spectrometer
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Resurrection of a Human Astrovirus–Neutralizing Antibody
Combining X-ray crystallography with mass spectrometry determines sequence and glycosylation of the Fab fragment of a potent HAstV-neutralizing mAb for therapeutic medicine.
https://www.thermofisher.com/blog/proteomics/resurrection-of-a-human-astrovirus-neutralizing-antibody/
Instrumentation includes an EASY-nLC 1000 nano ultra-high-performance liquid chromatograph (UHPLC)coupled to an Orbitrap Fusion Tribrid mass spectrometer or a Q Exactive mass spectrometer
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Aggregatibacter actinomycetemcomitans: A Healthy Mouth or Systemic Infection?
Mass spectrometric proteomics workflow in combination with molecular studies characterizes serotypic responses of A. actinomycetemcomitans to human serum in culture.
Study shows different responses according to serotype that could eventually explain why some infections cause periodontitits and others don't.
https://www.thermofisher.com/blog/proteomics/aggregatibacter-actinomycetemcomitans-a-healthy-mouth-or-systemic-infection/
Instrumentation includes an LTQ Orbitrap XL mass spectrometer.
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