Yes. Most of my regular players like them, so to cater for them... I usually add one.
My last campaign played with the concept... It was originally sold to the players as a quirky Urban Fantasy campaign, E.G. Treants were protesting plant rights and had created a picket forest around city hall and the weather forecast includes chance of rain, dead rising, prophecy, humidity, planar rifts and spontaneous, yet perfectly choreographed, dancing and singing. The reality was that it was set in the 14th Age and the entire city was a magically enslaved/manipulated living dungeon to create a living city that could grow and heal itself to replace Horizion which was destroyed towards the end of the 13th Age. I described the architecture in lovecraftian tones and made specific mention of how long it to travel between parts of the city despite the fact that it longer to explore traveling in the exact same path taken. I used it as a red herring with a corpse disappearing from its grave (the city ate it) when my players tried to reverse engineer potions and required the blood from the freshly dead after failing miserably at trying to identify any components in it. The campaign ended with a TPK when they managed to free the living dungeon by disrupting the spell that controlled it.
The prior uses were as a method of creating tension or giving the players a faster but more riskier/complicated option to reach their destination. I always described them with a few distinctly biological components. E.G. It had a heartbeat, the entire structure moved like it was alive, the wind regularly changed between warm and cold wind in opposite directions like it was breathing, barriers that acted like eyeballs with reactions to light, it would sneeze them out if they lit a torch inside, it was warm to the touch or recoiled to their touch, it would talk to them in either a quiet whisper or a deafening shout, the surface of the texture was too similar to scales to be a coincidence, It had blood vessels in the form of pipes that bleed a strange red mud when damaged or sometimes a place was described as bigger than on the map depicts because its grown since the map was made.