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Quantitative Proteomics
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Quantitative Proteomics

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The installation of the Orbitrap Q Exactive HF is in progress!
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Quantitative Proteomics

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New Book Chapter:
Brown LM. Quantitative Shotgun Proteomics with Data-Independent Acquisition and Traveling Wave Ion Mobility Spectrometry: A Versatile Tool in the Life Sciences
Adv Exp Med Biol. 2014;806:79-91. doi: 10.1007/978-3-319-06068-2_4.
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Quantitative Proteomics

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This PLOS blog highlighted our proteomics study as an excellent example of personalized medicine
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Quantitative Proteomics

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This series of posts today are references to some of our recent publications
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Alegre-Aguaron, E., Sampat, S.R., Xiong, J.C., Colligan, R.M., Bulinski, J.C., Cook, J.L., Ateshian, G.A., Brown, L.M., and Hung, C.T. 2014. Growth Factor Priming Differentially Modulates Components of the Extracellular Matrix Proteome in Chondrocytes and Synovium-Derived Stem Cells. PLoS ONE 9(2): e88053.
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Federica Parisi, Sara Riccardo, Sheri Zola, Carlina Lora, Daniela Grifoni, Lewis M. Brown, Paola Bellosta.  2013. dMyc expression in the fat body affects DILP2 release and increases the expression of the fat desaturase Desat1 resulting in organismal growth. Dev. Biol. 379:64-75 PMID: 23608455; PMC3712331.
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Quantitative Proteomics

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Wow, our new LC/MS has arrived!
UltiMate 3000 RSLCnano System with Orbitrap Q Exactive HF from Thermo Scientific

The Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer features an ultra-high-field Orbitrap analyzer which doubles its speed and resolution – more samples run and amazing data quality.

http://www.thermoscientific.com/en/product/q-exactive-hf-hybrid-quadrupole-orbitrap.html
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Ergel B, Gill ML, Brown L, Yu B, Palmer AG 3rd, Hunt JF.
Protein dynamics control the progression and efficiency of the catalytic reaction cycle of the Escherichia coli DNA-repair enzyme AlkB. J Biol Chem. 2014 Oct 24;289(43):29584-601
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Jin Yang, Yao Li, Lawrence Chan, Yi-Ting Tsai,Wen-Hsuan Wu,
Huy V. Nguyen, Chun-Wei Hsu, Xiaorong Li, Lewis M. Brown, Dieter Egli,
Janet R. Sparrow and Stephen H. Tsang. Validation of genome-wide association study (GWAS)-identified disease risk alleles with
patient-specific stem cell lines. Human Molecular Genetics, 2014 Advance Access published February 18, 2014
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Yang, Wan S., SriRamaratnam, R., Welsch, Matthew E., Shimada, K., Skouta, R., Viswanathan, Vasanthi S., Cheah, Jaime H., Clemons, Paul A., Shamji, Alykhan F., Clish, Clary B., Brown, Lewis M., Girotti, Albert W., Cornish, Virginia W., Schreiber, Stuart L., and Stockwell, Brent R. 2014. Regulation of Ferroptotic Cancer Cell Death by GPX4. Cell 156(1-2): 317-331.
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Nathan J. Weyand, Anne M. Wertheimer, Theodore R. Hobbs, Jennifer L. Sisko, Nyiawung A. Taku, Lindsay D. Gregston, Susan Clary, Dustin L. Higashi, Nicolas Biais, Lewis M. Brown, Shannon L. Planer, Alfred W. Legasse, Michael K. Axthelm, Scott W. Wong, Magdalene So. 2013. Neisseria infection of rhesus macaques as a model to study colonization, transmission, persistence and horizontal gene transfer. Proc Natl Acad Sci U S A. 110: 3059-3064. PMID: 23382234; PMC3581930.
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Deborah Moran, Trevor Cross, Lewis M. Brown, Ryan M. Colligan, David Dunbar. 2013. Data-independent acquisition (MSE) with ion mobility provides a systematic method for analysis of a bacteriophage structural proteome. Journal of Virol. 195:9-17
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Have them in circles
32 people
Matthew Collins's profile photo
Etudes Qualitative's profile photo
Israel Adedeji's profile photo
Erhua Zhang's profile photo
Bioproximity, LLC's profile photo
WON SUK YANG's profile photo
Bioproximity, LLC's profile photo
The Protein Society's profile photo
Omics Services's profile photo
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212-854-4470
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A Collaborative Laboratory at Columbia University
Introduction
The focus of the Quantitative Proteomics Center is the identification of proteins with differential quantitative expression in cells, tissues or in affinity purifications, and we have applied this technique employing mass spectrometry to many different research problems. We do projects with groups across all campuses of Columbia University and at other institutions across the nation. All internal and external research groups are treated equally.

Contact us to discuss your project.