Profile

Cover photo
208 followers|173,719 views
AboutPostsPhotosVideos

Stream

Cell Stem Cell

Shared publicly  - 
 
Dig into our FREE feature article: Wang lab reports that Dclk1 cells are rare, long-lived, and largely quiescent in the pancreas. Surprisingly, they are important for pancreatic regeneration after injury but pancreatic injury can also result in conversion of Dclk1 cells into highly efficient cancer-initiating cells. http://ow.ly/10Ahiu #stemcells
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
New online: Livesey lab investigates cortical neurogenesis with human and primate pluripotent cells in 2D and organoid systems, and find cell-autonomous differences in cortical progenitor proliferation before neurogenic differentiation. Their findings suggest that cortical size and cognitive ability between human and other animals result at least in part from species-specific clonal output during brain development. http://ow.ly/10jG2k
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
The latest Cell Press podcast tells the backstory of our recently published study on Zika virus infection of neural stem cells. Authors Guo-li Ming, Hongjun Song, and Hengli Tang explain how the collaboration developed. http://ow.ly/105kXl #stemcells
In this edition, we learn how the Zika virus affects fetal development, with Guo-li Ming, Hongjun Song, and Hengli Tang, Cell Stem Cell (00:00), how fungi may be used to create batteries, with Geoffrey Gadd, Current Biology (5:45), how organs can be mimicked with chip technology, ...
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
New Online: Alan D/Andrea and colleagues identify a new therapeutic target for treating Fanconi anemia. They find that inhibition of TGF-b rescues bone marrow failure in FA by promoting DNA repair in FA HSCs.
Here are some highlights and a summary of the paper:
•The hyperactive TGF-β pathway suppresses the survival of FA cells upon DNA damage
•TGF-β inhibition rescues proliferation defects of human and mouse FA HSCs
•Inhibition of the TGF-β pathway rescues bone marrow failure in FA mice
•TGF-β pathway inhibition promotes DNA repair in FA HSCs by increasing HR
Summary
Fanconi anemia (FA) is an inherited DNA repair disorder characterized by progressive bone marrow failure (BMF) from hematopoietic stem and progenitor cell (HSPC) attrition. A greater understanding of the pathogenesis of BMF could improve the therapeutic options for FA patients. Using a genome-wide shRNA screen in human FA fibroblasts, we identify transforming growth factor-β (TGF-β) pathway-mediated growth suppression as a cause of BMF in FA. Blocking the TGF-β pathway improves the survival of FA cells and rescues the proliferative and functional defects of HSPCs derived from FA mice and FA patients. Inhibition of TGF-β signaling in FA HSPCs results in elevated homologous recombination (HR) repair with a concomitant decrease in non-homologous end-joining (NHEJ), accounting for the improvement in cellular growth. Together, our results suggest that elevated TGF-β signaling contributes to BMF in FA by impairing HSPC function and may be a potential therapeutic target for the treatment of FA.#stemcells http://ow.ly/ZVm3e
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
New Online: Sean Morrison and colleagues show that Leptin/LepR signaling regulates bone marrow MSC adipogenesis and osteogenesis in response to diet and adiposity.
•Leptin/LepR signaling regulates osteogenesis by skeletal stem cells in bone marrow
•LepR signaling promotes adipogenesis and inhibits osteogenesis by BM stromal cells
•A high-fat diet promotes BM adipogenesis through LepR signaling in BM stromal cells
•LepR signaling in bone marrow mesenchymal stromal cells inhibits fracture healing
Summary
Skeletal stem cells (SSCs) that are the major source of osteoblasts and adipocytes in adult bone marrow express leptin receptor (LepR). To test whether LepR regulates SSC function, we conditionally deleted Lepr from limb bone marrow stromal cells, but not from the axial skeleton or hypothalamic neurons, using Prx1-Cre. Prx1-Cre;Leprfl/fl mice exhibited normal body mass and normal hematopoiesis. However, limb bones from Prx1-Cre;Leprfl/fl mice exhibited increased osteogenesis, decreased adipogenesis, and accelerated fracture healing. Leptin increased adipogenesis and reduced osteogenesis by activating Jak2/Stat3 signaling in bone marrow stromal cells. A high-fat diet increased adipogenesis and reduced osteogenesis in limb bones from wild-type mice, but not from Prx1-Cre;Leprfl/fl mice. This reflected local effects of LepR on osteogenesis and adipogenesis by bone marrow stromal cells and systemic effects on bone resorption. Leptin/LepR signaling regulates adipogenesis and osteogenesis by mesenchymal stromal cells in the bone marrow in response to diet and adiposity.#stemcells http://ow.ly/ZVle8
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
From our latest issue: Christine Mummery, Robert Passier, and Valerie Orlova review "Complex Tissue and Disease Modeling using hiPSCs."

The image here is a timeline of developments in the hiPSC field. In the review they cover advances starting from spontaneous and uncontrolled differentiation of mixed cell types via “embryoid bodies” toward controlled differentiation of specialized cells types in defined conditions that can be used for disease modeling and, finally, the development of complex structures and systems that recapitulate human tissues on a small scale, such as organoids and organ-on-chip technologies for disease modeling application. #stemcells http://ow.ly/Zw1Ds
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
Divergent lncRNAs Regulate Gene Expression and Lineage Differentiation in Pluripotent Cells

Hot off the presses! Xiaohua Shen and colleagues at Tsinghua University analyze the genome-wide distribution of divergent lncRNAs in pluripotent #stemcells and find that they are located near essential developmental genes and facilitate their expression. Check out the paper here: http://ow.ly/ZDIEs

Highlights:
•Genomic organization of divergent lncRNAs correlates strongly with regulatory genes
•Divergent lncRNAs regulate the expression of adjacent genes in pluripotent cells
•The lncRNA Evx1as binds locally to chromatin and promotes EVX1 transcription in cis
•Evx1as acts upstream of EVX1 to mediate mesendodermal lineage differentiation
Summary:
Divergent lncRNAs that are transcribed in the opposite direction to nearby protein-coding genes comprise a significant proportion (∼20%) of total lncRNAs in mammalian genomes. Through genome-wide analysis, we found that the distribution of this lncRNA class strongly correlates with essential developmental regulatory genes. In pluripotent cells, divergent lncRNAs regulate the transcription of nearby genes. As an example, the divergent lncRNA Evx1as promotes transcription of its neighbor gene, EVX1, and regulates mesendodermal differentiation. At a single-cell level, early broad expression of Evx1as is followed by a rapid, high-level transcription of EVX1, supporting the idea that Evx1as plays an upstream role to facilitate EVX1 transcription. Mechanistically, Evx1as RNA binds to regulatory sites on chromatin, promotes an active chromatin state, and interacts with Mediator. Based on our analyses, we propose that the biological function of thousands of uncharacterized lncRNAs of this class may be inferred from the role of their neighboring adjacent genes.
1
Add a comment...
Have them in circles
208 people
Celulas Madres's profile photo
Trends's profile photo
Sheida Shahraki's profile photo
Xytex Cryo International's profile photo
Stem Cells Craze's profile photo
Ariel Rinon's profile photo
Daryl Fallin (theDaryl)'s profile photo
Matej Buzgo's profile photo
Petter Lauritz Sand's profile photo

Cell Stem Cell

Shared publicly  - 
 
Our April issue went live yesterday - between CRISPR in iPSCs inducing gene silencing via interference, or restoring dystrophin expression via large scale deletion, and epigenetic mechanisms controlling regeneration (SWI/SNF) or hematopoietic stem cells (SIRT6/Wnt) we hope you will find something that peaks your interest! http://ow.ly/10sjZP #stemcells
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
New Online: Expression analysis highlights AXL as a candidate Zika virus entry receptor in neural stem cells.
The report, from Arnold Kriegstein and colleagues, uses single cell expression analysis of human radial glia, brain capillaries and microglia and finds they express high levels of the candidate receptor AXL. The receptor is also expressed in rodent and human cerebral organoid models, which may be used to study the mechanisms of ZIKV infection and its effects on brain development in future work.
Find out more about the study here: http://ow.ly/106Is2 #stemcells
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
New online from Hao Zhu and colleagues at UT Southwestern: Suppression of the SWI/SNF Component Arid1a Promotes Mammalian Regeneration.

•Arid1a loss results in improved regeneration after diverse liver injuries
•Arid1a overexpression impairs liver proliferation and regeneration
•Arid1a loss also improves tissue repair after ear hole punch
•Arid1a loss remodels chromatin, altering transcriptional access by C/EBPα and E2F4
Summary
Mammals have partially lost the extensive regenerative capabilities of some vertebrates, possibly as a result of chromatin-remodeling mechanisms that enforce terminal differentiation. Here, we show that deleting the SWI/SNF component Arid1a substantially improves mammalian regeneration. Arid1a expression is suppressed in regenerating tissues, and genetic deletion of Arid1a increases tissue repair following an array of injuries. Arid1a deficiency in the liver increases proliferation, reduces tissue damage and fibrosis, and improves organ function following surgical resection and chemical injuries. Hepatocyte-specific deletion is also sufficient to increase proliferation and regeneration without excessive overgrowth, and global Arid1a disruption potentiates soft tissue healing in the ear. We show that Arid1a loss reprograms chromatin to restrict promoter access by transcription factors such as C/ebpα, which enforces differentiation, and E2F4, which suppresses cell-cycle re-entry. Thus, epigenetic reprogramming mediated by deletion of a single gene improves mammalian regeneration and suggests strategies to promote tissue repair after injury.#stemcells http://ow.ly/ZVlBv
2
2
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
Are you going to be in the Boston area on Thursday May19th and are you interested in gender and science? If so, you're in luck! In collaboration with the Association for Women in Science, Cell Press is presenting a free LabLinks meeting: The Gender of Science and the Science of Gender.

Registration is free, but you need to sign up. http://ow.ly/ZDKEJ
1
Add a comment...

Cell Stem Cell

Shared publicly  - 
 
In their Perspective article from this month's Featured Five Reviews, Zena Werb and colleagues ask "How Essential Is the Niche in Regulating Stemness of Tumor Cells?"

Their conclusion is that the niche is critical to understanding how tumors form and progress into malignant disease. Since cancer stem cells survive many commonly employed cancer therapies, they also discuss the prospects for targeting the niche components and in some cases, they consider them to be preferable therapeutic targets to the tumor cells themselves. #cancer #stemcells http://ow.ly/Zw1rj
2
1
Add a comment...
People
Have them in circles
208 people
Celulas Madres's profile photo
Trends's profile photo
Sheida Shahraki's profile photo
Xytex Cryo International's profile photo
Stem Cells Craze's profile photo
Ariel Rinon's profile photo
Daryl Fallin (theDaryl)'s profile photo
Matej Buzgo's profile photo
Petter Lauritz Sand's profile photo
Contact Information
Contact info
Email
Story
Tagline
Cell Stem Cell is monthly journal from Cell Press launched in June 2007.
Introduction
Cell Stem Cell is an official affiliated journal of the International Society for Stem Cell Research (ICCSR), and is published by Cell Press in Cambridge, MA.  

We drive innovation in science by publishing exciting stem cell research and supporting investigators with resources and tools for connecting and collaborating with their peers.