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Sam White
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Sam White

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The final step before sequencing these 2bRAD libraries is to quantify them. Used the KAPA Illumina Quantification Kit (KAPA Biosystems) according to the manufacturer’s protocol. Made 1:4 dilutions of each library to use as template. Ran all samples,…
The final step before sequencing these 2bRAD libraries is to quantify them. Used the KAPA Illumina Quantification Kit (KAPA Biosystems) according to the manufacturer's protocol. Made 1:4 dilutions ...
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Sam White

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I recently ran gDNA isolated for geoduck and Olympia oyster genome sequencing, as well as gDNA isolated from the Olympia oyster reciprocal transplant experiment out on a Bioanalyzer (Agilent) using the DNA 12000 chips. The results from the chip were a bit…
I recently ran gDNA isolated for geoduck and Olympia oyster genome sequencing, as well as gDNA isolated from the Olympia oyster reciprocal transplant experiment out on a Bioanalyzer (Agilent) using...
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Sam White

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Continuing with the RAD-seq library prep. Following the Meyer Lab 2bRAD protocol. Prior to generating full-blown libraries, we needed to run a “test-scale” PCR to identify the minimum number of cycles needed to produce the intended product size (166bp). I…
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Sam White

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Earlier today, I created dilution series of the following two linearized plasmids to develop qPCR assays: pCR2.1/RLOv_DNA_helicase pCR2.1/RLOv_head_to_tail Master mix calcs: 20151106 – qPCR RLOv Standard Curves All samples were run in triplicate on a…
Earlier today, I created dilution series of the following two linearized plasmids to develop qPCR assays: pCR2.1/RLOv_DNA_helicase pCR2.1/RLOv_head_to_tail Master mix calcs: 20151106 - qPCR RLOv St...
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Sam White

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Quantified the linearized RLOv plasmids using the Quant-It DNA BR Kit (Invitrogen) according to the manufacturer’s protocol. Standards (10μL each of 0, 5, 10, 20, & 40ng) were run in triplicate. Linearized plasmids were quantified in replicates of six.…
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Sam White

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Comparison of three different approaches to using the E.Z.N.A. Mollusc Kit: Fresh isolations by me Fresh isolations by Mrunmayee Isolations from tissue frozen in buffer by me Results: Bioanalyzer Data File (XAD file): 2100 expert_DNA…
Comparison of three different approaches to using the E.Z.N.A. Mollusc Kit: Fresh isolations by me Fresh isolations by Mrunmayee Isolations from tissue frozen in buffer by me Results: Bioanalyzer D...
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Sam White

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Extracted the PCR products from the gel slices from 20151113 using the QIAQuick Gel Extraction Kit (Qiagen) according to the manufacturer’s protocol. Substituted MiniElute columns so that I could elute with a smaller volume than what is used in the…
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Sam White

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Continuing with the RAD-seq library prep. Following the Meyer Lab 2bRAD protocol. After determining the minimum number of PCR cycles to run to generate a visible, 166bp band on a gel yesterday, ran a full library “prep scale” PCR.   REAGENT SINGLE…
Continuing with the RAD-seq library prep. Following the Meyer Lab 2bRAD protocol. After determining the minimum number of PCR cycles to run to generate a visible, 166bp band on a gel yesterday, ran...
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Sam White

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Ran probe-labeling PCRs to use in in-situ hybridization (ISH) using the PCR DIG Probe Sysnthesis Kit (Roche). Generated PCR probes for using the following BamHI-linearized plasmids: pCR2.1/RLOv_membrane_gene_1 pCR2.1/RLOv_membrane_gene_1…
Ran probe-labeling PCRs to use in in-situ hybridization (ISH) using the PCR DIG Probe Sysnthesis Kit (Roche). Generated PCR probes for using the following BamHI-linearized plasmids: pCR2.1/RLOv_mem...
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Sam White

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Set up standard curve dilutions to use for qPCRs with the following two linearized plasmids: pCR2.1/RLOv_DNA_helicase pCR2.1/RLOv_head_to_tail Used a spreadsheet developed by Nate many moons ago to determine necessary volumes based on plasmid size to…
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Sam White

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Set up restriction digestions to linearize the pCR2.1/RLOv plasmids in preparation for ISH probes and qPCR standard curves. Used BamHI (NEB), since it doesn’t cut in any of the RLOv sequences and cuts one time in pCR2.1-TOPO (Invitrogen). PLASMID Vol for…
Set up restriction digestions to linearize the pCR2.1/RLOv plasmids in preparation for ISH probes and qPCR standard curves. Used BamHI (NEB), since it doesn't cut in any of the RLOv sequences and c...
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Sam White

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Quantified the following samples with the Roberts Lab NanoDrop1000 (ThermoFisher) and assessed DNA integrity on the Seeb Lab Bioanalyzer 2100 (Agilent) using the DNA 12000 chip assay: geoduck gDNA for genome sequencing Olympia oyster gDNA for genome…
Quantified the following samples with the Roberts Lab NanoDrop1000 (ThermoFisher) and assessed DNA integrity on the Seeb Lab Bioanalyzer 2100 (Agilent) using the DNA 12000 chip assay: geoduck gDNA ...
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In their circles
151 people
Have them in circles
123 people
Harry Podschwit's profile photo
Claire Horner-Devine's profile photo
Kevin Jeong's profile photo
Dorcas Buabeng's profile photo
Zac Halls's profile photo
Hema Chawla's profile photo
Faith Lynn's profile photo
Jonathan Allen's profile photo
Moga Saewy's profile photo
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I like playing video games and eating Kraft macaroni & cheese w/hotdogs.  Oh, and I also like Phish!
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