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Sam White
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Sam White

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We had our first true test of the Synology RAID redundancy with our Synology 1812+ server (Owl). One of the hard drives (HDD) failed. All of the other drives were fine, the data was intact and we had a new replacement HDD on hand. However, there was one…
We had our first true test of the Synology RAID redundancy with our Synology 1812+ server (Owl). One of the hard drives (HDD) failed. All of the other drives were fine, the data was intact and we h...
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Sam White

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After designing new primers for use in Withering Syndrome phage (RLOv) identification on 20150706, ran Primer-BLAST via NCBI’s website to assess primer specificity. Ran Primer-BLAST with each primer set against the NCBI nr Viruses (Tax ID:10239 ) and…
After designing new primers for use in Withering Syndrome phage (RLOv) identification on 20150706, ran Primer-BLAST via NCBI's website to assess primer specificity. Ran Primer-BLAST with each prime...
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Sam White

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Isolated RNA from Jake’s Ostrea lurida ctenidia samples that had been subjected to mechanical stress (from 20150422). Despite the indication in this notebook, the samples had not been previously homogenized in RNAzol RT. I thawed the samples, homogenized…
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Prepared sterile, 1.0mL aliquots of SsoFast EvaGreen Supermix (received today) in 2.0mL screw cap tubes. All aliquots were dated and stored @ -20C in the “PCR Supplies” box. BioRad Lot#: 730003517
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Sam White

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Ran qPCRs on remaining 2011 water filter DNA samples. All samples were from The Cultured Abalone farm, but some samples are abbreviated “CAB”, while others “TCA”. Samples were extracted 20131001 and 20131120. Master mix calcs: 20150702 – qPCR WS Ab Endo…
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Sam White

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Before we check out this month’s goals, let’s have a quick review of last month’s goals and which, if any, I was able to accomplish:  From June 2015: Geoduck Reproductive Development Transcriptomics Done. Data from this was received 20150629. BS-Seq…
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Sam White

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Quantified the RNA I isolated from Jake’s samples on 20150715 and 20150710 using the Roberts Lab NanoDrop1000 (ThermoFisher).       Overall, the yields are good. The 260/280 ratios are mediocre. Will proceed with DNase treatment. DNased 1.5ug of RNA from…
Quantified the RNA I isolated from Jake's samples on 20150715 and 20150710 using the Roberts Lab NanoDrop1000 (ThermoFisher).       Overall, the yields are good. The 260/280 ratios a...
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Sam White

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Stan Langevin recently annotated the Withering Syndrome (WS) bacteriophage genome he previously assembled. Additionally, after discussing with Carolyn, they decided on two new potential qPCR targets and three potential in-situ hybridization (ISH) targets.…
Stan Langevin recently annotated the Withering Syndrome (WS) bacteriophage genome he previously assembled. Additionally, after discussing with Carolyn, they decided on two new potential qPCR target...
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Sam White

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Isolated RNA from Jake’s Ostrea lurida ctenidia samples that had been subjected to mechanical stress (from 20150422). Despite the indication in this notebook, the samples had not been previously homogenized in RNAzol RT. I thawed the samples, homogenized…
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I’ve been tweaking a shell script (notebook_backups.sh) to use the shell program wget to retrieve fully functional HTML versions of our online notebooks for offline viewing. I had been planning on setting up a cron job to automatically run this script on…
I've been tweaking a shell script (notebook_backups.sh) to use the shell program wget to retrieve fully functional HTML versions of our online notebooks for offline viewing. I had been planning on ...
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Sam White

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Ran a PCR to obtain luciferase DNA for sequencing. Used sea pen gDNA extracted by Jonathan on 20150527. Primers: SRID Name 1604 Rr_46_65F 1603 Rr_887_868R Master mix calcs are here: 20150702_seapen_PCR Cycling params: 95C – 10mins 95C – 15s 55C – 15s 72C…
Ran a PCR to obtain luciferase DNA for sequencing. Used sea pen gDNA extracted by Jonathan on 20150527. Primers: SRID Name 1604 Rr_46_65F 1603 Rr_887_868R Master mix calcs are here: 20150702_seapen...
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Jake and Steven recently noticed localized “hot spots” on most of Jake’s recent qPCRs, where higher levels of fluorescence were consistently showing up in interior portions of the plates than the outer portion of the plates. Ordered 5nmol of 6-FAM T10…
Jake and Steven recently noticed localized "hot spots" on most of Jake's recent qPCRs, where higher levels of fluorescence were consistently showing up in interior portions of the plates than the o...
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Thanks for the info +Anne Baxter​. Unfortunately, we don't use film, just strip caps.
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In their circles
150 people
Have them in circles
122 people
Robert Cuomo's profile photo
Alyssa Lewis's profile photo
Charles Dueber's profile photo
Neil Johnson's profile photo
David Rhoads's profile photo
Shariq Ahmad's profile photo
Lisa Crosson's profile photo
Rajani George's profile photo
Faith Lynn's profile photo
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I like playing video games and eating Kraft macaroni & cheese w/hotdogs.  Oh, and I also like Phish!
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The Dude abides...