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Sam White
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Sam White

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I’ve been tweaking a shell script (notebook_backups.sh) to use the shell program wget to retrieve fully functional HTML versions of our online notebooks for offline viewing. I had been planning on setting up a cron job to automatically run this script on…
I've been tweaking a shell script (notebook_backups.sh) to use the shell program wget to retrieve fully functional HTML versions of our online notebooks for offline viewing. I had been planning on ...
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Sam White

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Ran a PCR to obtain luciferase DNA for sequencing. Used sea pen gDNA extracted by Jonathan on 20150527. Primers: SRID Name 1604 Rr_46_65F 1603 Rr_887_868R Master mix calcs are here: 20150702_seapen_PCR Cycling params: 95C – 10mins 95C – 15s 55C – 15s 72C…
Ran a PCR to obtain luciferase DNA for sequencing. Used sea pen gDNA extracted by Jonathan on 20150527. Primers: SRID Name 1604 Rr_46_65F 1603 Rr_887_868R Master mix calcs are here: 20150702_seapen...
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Sam White

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Jake and Steven recently noticed localized “hot spots” on most of Jake’s recent qPCRs, where higher levels of fluorescence were consistently showing up in interior portions of the plates than the outer portion of the plates. Ordered 5nmol of 6-FAM T10…
Jake and Steven recently noticed localized "hot spots" on most of Jake's recent qPCRs, where higher levels of fluorescence were consistently showing up in interior portions of the plates than the o...
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Thanks sam for checking this out.
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Sam White

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Submitted a plate of purified PCR products (PCR products prepared by Jake on 20150623) that Jake set up yesterday, to the UW High-Throughput Genomics Center for Sanger sequencing. Plate layout is here (Google Sheet): sequence_log Order #:112442
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Sam White

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Finished measuring Ostrea lurida larvae using NIS-Elements BR (10x objective and 10x nosepiece setting in software). That’s 2,620 larvae measured! NOTE: Robyn previously measured 33 tubes worth of larvae, but I’m not certain where that data was saved. All…
Finished measuring Ostrea lurida larvae using NIS-Elements BR (10x objective and 10x nosepiece setting in software). That's 2,620 larvae measured! NOTE: Robyn previously measured 33 tubes worth of ...
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Continued measurements of larvae samples from yesterday. Measured length (hinge to growing edge) and width of 30 oysters from each tube. The measurement data can be found here (Google Sheet): FHL_Oly_larvae_measurements Here’s the list of tubes that were…
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Sam White

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Ran qPCRs on remaining 2011 water filter DNA samples. All samples were from The Cultured Abalone farm, but some samples are abbreviated “CAB”, while others “TCA”. Samples were extracted 20131001 and 20131120. Master mix calcs: 20150702 – qPCR WS Ab Endo…
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Before we check out this month’s goals, let’s have a quick review of last month’s goals and which, if any, I was able to accomplish:  From June 2015: Geoduck Reproductive Development Transcriptomics Done. Data from this was received 20150629. BS-Seq…
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Received notification that the samples sent on 20150601 for RNAseq were completed. Downloaded the following files from the GENEWIZ servers using FileZilla FTP and stored them on our server (owl/web/nightingales/P_generosa):…
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Master mix calcs are here: 20150622 – qPCR WS Ab Endo H2O Filters All samples were run in duplicate. See the qPCR Report (in Results below) for plate layout, cycling params, etc. Standard curve was p18RK7 from 20120731. Baseline threshold set to 580,…
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Continued measuring Ostrea lurida larvae using NIS-Elements BR (10x objective and 10x nosepiece setting in software). All measurements can be found here (Google Sheet): FHL_Oly_larvae_measurements Measured 30 larvae from each tube of the following…
Continued measuring Ostrea lurida larvae using NIS-Elements BR (10x objective and 10x nosepiece setting in software). All measurements can be found here (Google Sheet): FHL_Oly_larvae_measurements ...
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Performed reverse transcription on the Olympia oyster DNased RNA from the control samples and the 1hr heat shock samples of Jake’s project. To accommodate the large numbers of anticipated genes to be targeted in subsequent qPCRs, I prepared 100μL…
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In their circles
150 people
Have them in circles
123 people
Mike Muellner's profile photo
Claire Olson's profile photo
Robert Cummings's profile photo
Anne White's profile photo
Patrick Schmitt's profile photo
Moga Saewy's profile photo
Jane White's profile photo
Daniel Williams's profile photo
Shariq Ahmad's profile photo
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Introduction
I like playing video games and eating Kraft macaroni & cheese w/hotdogs.  Oh, and I also like Phish!
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The Dude abides...