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Sam White
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Sam White

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Ran 0.8% agarose 1x modified TAE gel stained with EtBr to assess the integrity of geoduck gDNA (from 20150828) and Olympia oyster gDNA (from 20150901). Ran 500ng of each sample: Geoduck adductor muscle: 4.87μL Geoduck foot: 4.50μL Oly adductor muscle:…
Ran 0.8% agarose 1x modified TAE gel stained with EtBr to assess the integrity of geoduck gDNA (from 20150828) and Olympia oyster gDNA (from 20150901). Ran 500ng of each sample: Geoduck adductor mu...
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Sam White

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The system was previously set to use Steven’s Comcast SMTP server. Sending a test email from Eagle failed, indicating authentication failure. I changed this to use the University of Washington’s email server for outgoing messages. Here’s how… In the…
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Sam White

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Helped Dan with oyster larval setting. Went to Taylor Shellfish Hatchery in Quilcene, WA with Kristin Saksa to collect and count larvae. After larvae were collected, counted and incubated (refrigerator for 1hr), we transported the larvae to the Manchester…
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Sam White

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Helped Dan create oyster down-wellers (silos) of 20μm, 50μm, and 100μm mesh sizes for housing oyster larvae. He needs ~200 of each. PVC had already been cut to size and drilled. Mesh needed to be rubberbanded and then fixed to the PVC tubes using…
Helped Dan create oyster down-wellers (silos) of 20μm, 50μm, and 100μm mesh sizes for housing oyster larvae. He needs ~200 of each. PVC had already been cut to size and drilled. Mesh needed to be r...
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Sam White

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Helped Dan create oyster down-wellers (silos) of 20μm, 50μm, and 100μm mesh sizes for housing oyster larvae. He needs ~200 of each. PVC had already been cut to size and drilled. Mesh needed to be rubberbanded and then fixed to the PVC tubes using…
Helped Dan create oyster down-wellers (silos) of 20μm, 50μm, and 100μm mesh sizes for housing oyster larvae. He needs ~200 of each. PVC had already been cut to size and drilled. Mesh needed to be r...
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Prepared sterile, ~1.5mL aliquots of SsoFast EvaGreen Supermix (received 20150810) in 2.0mL screw cap tubes. All aliquots were dated and stored @ -20C in the “PCR Supplies” box. BioRad Lot#: 730003517
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In their circles
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124 people
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Sam White

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Genomic DNA Isolation - Olympia oyster adductor musle & mantle

Isolated gDNA from Ostrea lurida (Olympia oyster) adductor muscle & mantle samples collected by Brent & Steven on 20150812 using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek) according to the manufacturer’s protocol, with the following adjustments: 29.8mg…
Isolated gDNA from Ostrea lurida (Olympia oyster) adductor muscle & mantle samples collected by Brent & Steven on 20150812 using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek) according to the...
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Sam White

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Isolated gDNA from Panopea generosa (geoduck) adductor muscle & foot samples collected by Brent & Steven on 20150811 using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek) according to the manufacturer’s protocol, with the following adjustments: 41.8mg of…
Isolated gDNA from Panopea generosa (geoduck) adductor muscle & foot samples collected by Brent & Steven on 20150811 using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek) according to the manuf...
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Received vials of C.maximum (a vermetid gastropod) from Craig Osenberg. Topped off samples with either 100% ethanol or 70% ethanol, depending on sample requirements (specified on vial labels) and stored in the flammables cabinet in FSH 240.  
Received vials of C.maximum (a vermetid gastropod) from Craig Osenberg. Topped off samples with either 100% ethanol or 70% ethanol, depending on sample requirements (specified on vial labels) and s...
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Sam White

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Helped Dan create oyster down-wellers (silos) of 20μm, 50μm, and 100μm mesh sizes for housing oyster larvae. He needs ~200 of each. PVC had already been cut to size and drilled. Mesh needed to be rubberbanded and then fixed to the PVC tubes using…
Helped Dan create oyster down-wellers (silos) of 20μm, 50μm, and 100μm mesh sizes for housing oyster larvae. He needs ~200 of each. PVC had already been cut to size and drilled. Mesh needed to be r...
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A box with the above title was established in the -20C in FTR 209 containing the following: Thermo Scientific AlfI: ER1801 NEB T4 DNA ligase, 50 μL: M0202S NEB 10 mM ATP, 1 mL: P0756S dNTPs (10 mM each) NEB Q5 Taq Polymerase, 100 units: M0491S Oligos…
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Performed reverse transcription on the Olympia oyster DNased RNA from the 1hr post-mechanical stress samples from Jake’s project. To accommodate the large numbers of anticipated genes to be targeted in subsequent qPCRs, I prepared 100μL reactions…
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In their circles
150 people
Have them in circles
124 people
Lisa Crosson's profile photo
Meghan Taylor's profile photo
Bridget Johnson's profile photo
Jane White's profile photo
Steven Roberts's profile photo
Brent Vadopalas's profile photo
Anne Baxter's profile photo
Dorcas Buabeng's profile photo
Robert Cummings's profile photo
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I like playing video games and eating Kraft macaroni & cheese w/hotdogs.  Oh, and I also like Phish!
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