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Sam White
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Sam White

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After the previous round of sequencing analysis, decided to sequence a couple of additional clones from each of the three groups: 16s, EHR, EUB. Submitted ~500ng (3μL) of clones #2 & #3 (C2 & C3) from each group to GENEWIZ for Sanger sequencing (Order #:…
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Sam White

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Used up the remainder of the one positive control gDNA that worked with all the primers in yesterday’s reaction (H.crach_h-1fg_intron, H.iris_actin_intron, H.crach_16s), so need to find a new set of gDNA to use for future positive controls. qPCR plate…
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Sam White

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Our sequencing data (Illumina HiSeq2500, 100SE) for this project has completed by Univ. of Oregon Genomics Core Facility (order number 2212). Samples sequenced/pooled for this run: Sample Treatment Barcode 400ppm 400ppm GCCAAT 1000ppm 1000ppm CTTGTA   All…
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Sam White

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Performed reverse transcription with 307ng of each DNased withering syndrome RNA from earlier today using Promega reagents (M-MLV, random primers) according to the manufacturer’s recommendations in the M-MLV product insert. Reverse transcription calcs are…
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Sam White

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Withering syndrome RNA, extracted from water filters, was confirmed via qPCR to contain detectable quantities of withering syndrome DNA. Before proceeding to make cDNA, residual gDNA carryover from the RNA extraction needs to be removed. Performed DNase…
Withering syndrome RNA, extracted from water filters, was confirmed via qPCR to contain detectable quantities of withering syndrome DNA. Before proceeding to make cDNA, residual gDNA carryover from...
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Sam White

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"Eater Inside: Inside Taylor Shellfish's Oyster Bar Near the Space Needle" http://feedly.com/e/W_7ktCyA
The wait is over: Taylor Shellfish Oyster Bar opens this very day in Lower Queen Anne. The bright and shiny sibling of Taylor's three-year-old Melrose Market oyster bar seats...
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Sounds like something we should get together some time and check out. 
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Sam White

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This was done to really test the detection methods we’re using for gDNA contamination in our qPCRs. qPCR plate layout/set up is here. Anneal temp 50C. It should be noted that the 07:12-08 RNA was diluted 1:10 before adding to the rxns to make it similar…
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Sam White

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The qPCR on withering syndrome water filter cDNA that I ran earlier today didn’t amplify in any samples, and I neglected to run a positive control primer set on the cDNA to verify that the reverse transcription was successful. Ran a qPCR using universal…
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Sam White

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Ran qPCR on withering syndrome water filter cDNA from yesterday. Master mix calcs are here: 20150319 – qPCR WSN cDNA Ran p18RK7 standard curve from 20120731. All samples were run in duplicate. Plate layout, cycling params, etc. can be found in the qPCR…
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Sam White

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Ran qPCR on DNased withering syndrome RNA to verify there’s no residual gDNA, prior to performing reverse transciption. Master mix calcs are here: 20150318 – qPCR WSN DNased RNA Test Used 1μL of template. This was based on using 17.5μL of DNased RNA in…
Ran qPCR on DNased withering syndrome RNA to verify there's no residual gDNA, prior to performing reverse transciption. Master mix calcs are here: 20150318 - qPCR WSN DNased RNA Test Used 1μL of te...
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Sam White

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"Learn Regular Expressions with RegExr" http://feedly.com/e/L0kIK9st
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Sam White

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Americans die in smaller portions each year, but what kills us is changing.
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At first I thought, "hey another visualization that old people die at a faster rate than young people" but there's some cool stuff in here. I was also proud that I noticed that 25-44yo spike in mortality rate in the mid-90's before they called out the AIDS explanation.
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In their circles
150 people
Have them in circles
121 people
Sean Egan's profile photo
Claire Olson's profile photo
Brent Vadopalas's profile photo
Halley Froehlich's profile photo
Steven Roberts's profile photo
Neil Johnson's profile photo
Rainier Wardach's profile photo
Etilet Maipi's profile photo
Emma Timmins-Schiffman's profile photo
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I like playing video games and eating Kraft macaroni & cheese w/hotdogs.  Oh, and I also like Phish!
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