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Michael Pfaffl
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9th Gene Quantification Event -- qPCR dPCR & NGS 2019
Symposium & Industrial Exhibition & Application Workshops
Main topics: Next Generation Biomarkers -- Liquid Biopsy, Multi-Omics, MicroGenomics
main.qPCR-dPCR-NGS-2019.net
#GQ2019
18-22 March 2019, in Freising-Weihenstephan,
School of Life Sciences, Technical University of Munich, Weihenstephan, Germany
Download Event Announcement Announcement.qPCR-dPCR-NGS-2019.net

Impressions from a great GQ2017 symposium https://www.youtube.com/watch?v=PCpwDL3ArD0

Please register and submit your scientific contribution via our online registration tool Registration.qPCR-dPCR-NGS-2019.net

Symposium Sessions Sessions.qPCR-dPCR-NGS-2019.net

• Main topic: Next Generation Biomarkers
• Main topic: Liquid Biopsy & Circulating Nucleic Acids
• Main topic: Multi-Omics & Integrative Big Data Analysis
• Main topic: MicroGenomics & Single-Cells Diagnostics
• ISEV Satellite Session – EV & Exosome Diagnostic Biomarkers ( to be confirmed! )
• Digital PCR
• Non-coding RNAs -- microRNA, isomiRs, small RNAs, long non-coding RNAs
• Next Generation Sequencing (NGS)
• qPCR & NGS data analysis
• Advanced Molecular Diagnostics in Life Science
• MIQE & QM & Standardisation Strategies in Molecular Diagnostics
• Technical Lunch-Time Seminars
• Submission deadline is 15th January 2019

Post has shared content
9th Gene Quantification Event -- qPCR dPCR & NGS 2019
Symposium & Industrial Exhibition & Application Workshops
Main topics: Next Generation Biomarkers -- Liquid Biopsy, Multi-Omics, MicroGenomics
main.qPCR-dPCR-NGS-2019.net
#GQ2019
18-22 March 2019, in Freising-Weihenstephan,
School of Life Sciences, Technical University of Munich, Weihenstephan, Germany
Download Event Announcement Announcement.qPCR-dPCR-NGS-2019.net

Impressions from a great GQ2017 symposium https://www.youtube.com/watch?v=PCpwDL3ArD0

Please register and submit your scientific contribution via our online registration tool Registration.qPCR-dPCR-NGS-2019.net

Symposium Sessions Sessions.qPCR-dPCR-NGS-2019.net

• Main topic: Next Generation Biomarkers
• Main topic: Liquid Biopsy & Circulating Nucleic Acids
• Main topic: Multi-Omics & Integrative Big Data Analysis
• Main topic: MicroGenomics & Single-Cells Diagnostics
• ISEV Satellite Session – EV & Exosome Diagnostic Biomarkers ( to be confirmed! )
• Digital PCR
• Non-coding RNAs -- microRNA, isomiRs, small RNAs, long non-coding RNAs
• Next Generation Sequencing (NGS)
• qPCR & NGS data analysis
• Advanced Molecular Diagnostics in Life Science
• MIQE & QM & Standardisation Strategies in Molecular Diagnostics
• Technical Lunch-Time Seminars
• Submission deadline is 15th January 2019
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9th Gene Quantification Event -- qPCR dPCR & NGS 2019
9th Gene Quantification Event -- qPCR dPCR & NGS 2019 Symposium & Industrial Exhibition & Application Workshops Main topics: Next Generation Biomarkers -- Liquid Biopsy, Multi-Omics, MicroGenomics http://main.qPCR-dPCR-NGS-2019.net #GQ2019 18-22 March 2019,...
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Liquid Biopsies -- EMBL COURSE

Liquid biopsies have the advantage of being largely non-invasive and providing a vast amount of information for the detection and monitoring of diseases such as cancer. This course will provide hands-on experience in the most common techniques to detect and analyse circulating tumor cells (CTCs), cell free tumor DNA (ctDNA) and/or extracellular vesicles (exosomes) while also bringing in clinical aspects. Participants will have the opportunity to learn all aspects ranging from proper sampling through error free sequencing to data analysis.

Audience
This course targets young scientists entering the field of liquid biopsies and wanting to learn about the benefits and limitations of the approach.

Modules/Resources
- Hands-on experience on ultra-sensitive mutation detection using sequencing
- Application of digital PCR to the liquid biopsy workflow
- Protein analysis in liquid biopsis
- Data analysis

Learning Outcomes
Participants will learn about the complete workflow in liquid biopsies, from sample preparation to data analysis and gain an overview of the current methods. Best practices, pitfalls as well as do's and don'ts will be discussed. After the course, participants should be able to assess the best methods for their application as well as design their experiments.
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New surveillance concepts in food safety in meat producing animals: The advantage of high throughput ‘omics’ technologies
Michael W. Pfaffl & Irmgard Riedmaier-Sprenzel
Asian-Australas J Anim Sci > Accepted Articles
Review paper [Accepted] Published online May 31, 2018.
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Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing.
Dominik Buschmann , Benedikt Kirchner , Stefanie Hermann , Melanie Märte , Christine Wurmser , Florian Brandes , Stefan Kotschote, Michael Bonin, Ortrud K. Steinlein , Michael W. Pfaffl , Gustav Schelling & Marlene Reithmair
Published online 04 Jun 2018
Journal of Extracellular Vesicles 2018, Volume 7, Issue 1
https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1481321
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Yup, in 17 years more than 22,000 citations — A new mathematical model for relative quantification in real-time RT–PCR | Nucleic Acids Research 2001 — https://academic.oup.com/nar/article/29/9/e45/2384081
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Stimulated enrichment of Clostridium difficile specific IgA in mature cow's milk.
Christiane Schmautz, Maria Hillreiner, Ines Ballweg, Michael W. Pfaffl, Heike Kliem
PlosOne -- published: April 25, 2018
https://doi.org/10.1371/journal.pone.0195275
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The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression.
Rreze M. Gecaj, Corina I. Schanzenbach, Benedikt Kirchner, Michael W. Pfaffl, Irmgard Riedmaier, Ry Y. Tweedie-Cullen, and Bajram Berisha
Front Genet. 2017; 8: 213.
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