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The Pathologist
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PTEN mRNA detection by chromogenic, RNA
in-situ technologies: A reliable alternative to PTEN Immunohistochemistry

January 28th, 2016 11:00 am EST | 02:00 pm GMT | 03:00 pm CET

Immunohistochemical staining for Phosphatase and Tensin Homolog (PTEN) does not have either an acceptable standard protocol or concordance of scoring between pathologists. Evaluation of PTEN mRNA with a unique and verified sequence probe may offer a realistic alternative providing a robust and reproducible protocol. In this study we have evaluated an in-situ hybridization (ISH) protocol for PTEN mRNA using RNAscope® technology and compared it with a standard protocol for PTEN immunohistochemistry (IHC). mRNA detection of PTEN or other biomarkers, for which optimal or standardized immunohistochemical techniques are not available, represents a means by which heterogeneity of expression within focal regions of tumour can be explored with more confidence.

Learning Objectives of Webinar

1. How the PTEN expression profile in various cancer types can be used as a prognostic marker.

2. How the detection of PTEN mRNA expression in tissue sections preserving the morphological context, and therefore the heterogeneity of expression within focal regions of various tumour types, can be explored.

3. Why RNAscope® in-situ hybridization (ISH) is a good alternative for the detection of biomarkers, such as PTEN, when there is no optimal or standardized immunohistochemical techniques available.

http://view6.workcast.net/register?pak=9626850333797439
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Great presentation at this week’s CAP meeting in the US. Check out mention of The Pathologist magazine at 19 mins!
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Our latest newsletter for those who missed it: http://ow.ly/SJB6e  
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New analytical tools could now make RNA a useful tool in the pathologist’s kit http://ow.ly/SJGI6  
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#ThePathologist Power list 2015 - Find out more & nominate at: http://ow.ly/RKqTk   or tweet @pathologistmag 
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Less than two weeks left to nominate for The Pathologist’s Power List 2015!
Time is running out to nominate the people you admire most in laboratory medicine. Don’t miss the opportunity to see them recognized for everything they’ve accomplished!
-Do you want your area of lab medicine to gain more publicity?
-Do you feel the contributions of those you admire in lab medicine are going unnoticed?
-Do you think your profession is unfairly stereotyped?
-Do you want to shine a light on role models that are inspiring change in lab medicine?

If the answer to any of those things is “yes,” now is your chance to make sure it happens.
Ready to nominate, or want to know more about the process? Visit:
https://thepathologist.com/issues/0615/its-time-to-cast-your-vote-the-pathologist-power-list/
(Wondering if this applies to you? We want to see nominations for all lab medicine specialties – from microbiology to microscopy, from clinical biochemistry to molecular medicine, and everything in between – anyone who is revolutionizing their field.)
And if you share this with your friends, you never know – you may end up on the list yourself!
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ICYMI- As science fiction technology inches closer to reality, teams across the globe are competing to create the world’s first medical tricorder http://ow.ly/RS4ha
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ICYMI- Microchip technology can detect very low quantities of lung cancer biomarkers in breath – and may offer a new noninvasive screening option http://ow.ly/RS4ac
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