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Index Sorting - From FACSDiVa to FlowJo
We recently upgraded our FACSAria to FACSDiVa 8 running on Windows 7 primarily for the ability to do index sorting. Getting used to a brand new set of DiVa issues and quirks has been difficult, but we soldiered on nonetheless. After scouring the web for res...
We recently upgraded our FACSAria to FACSDiVa 8 running on Windows 7 primarily for the ability to do index sorting. Getting used to a brand new set of DiVa issues and quirks has been difficult, but we soldiered on nonetheless. After scouring the web for resources on both index sorting and ...
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Core Facility Acknowledgment Accounting 101 - How to make sure your work is being recognized.
BioTechniques Article on SRL Attribution A recent article in Biotechniques has spurred some interesting discussions in the Academic Core Facility (or as we cytometry cores like to call them, Shared Resource Laboratories - SRLs) world. The gist of the articl...
A recent article in Biotechniques has spurred some interesting discussions in the Academic Core Facility (or as we cytometry cores like to call them, Shared Resource Laboratories - SRLs) world. The gist of the article states that all too often core facilities are not properly acknowledged in ...
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We're moving!

Effective June 1st, 2014, our new address will be:

UCFlow, University of Chicago
924 E. 57th St. Room R022
Chicago, IL 60637

All other contact information will remain the same.
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21st Century Learning using an Ancient Model Applied to Flow Cytometry Training
There are so many things I'd love to learn.  I always imagined myself playing the guitar, or executing a no-hands backflip, or even writing a mobile application, but so far, I still cannot do any of those things. Of course, each of these things are certainl...
There are so many things I'd love to learn. I always imagined myself playing the guitar, or executing a no-hands backflip, or even writing a mobile application, but so far, I still cannot do any of those things. Of course, each of these things are certainly in the realm of possibilities for me.
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Ready to throw some money down on a new cytometer?  Read my 10 tips for purchasing your next cytometer here.
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The Avalon Cell Sorter developed by Propel-Labs has been purchased by Bio-Rad.  The deal, which will allow Propel to reach its market and keep up with demand, gives Bio-Rad the 'rights' to the instrument in exchange for an undisclosed dollar amount.  Now, all the questions about how Propel-Labs will handle service, sales, and manufacturing out of their tiny facilities in Ft. Collins, CO should be answered.  

Seems like a good move for Propel, and a very good move for Bio-Rad, which contrary to the sounds of it's name doesn't do too much in the cell-based life sciences.  Bio-Rad is actually opening up a brand new Flow Cytometry division, which I could only imagine will include hardware and reagents.  It seems to me that they might be in the 'buying' mood.  Perhaps they'll follow suit with Affymetrix and pick up an antibody company (like BioLegend, for example).  We'll see...

Here's the new page for the S3 Cell Sorter (formerly the Avalon) with it's new paint job:  http://www.bio-rad.com/prd/en/US/LSR/PDP/MC3PU4E8Z/S3-Cell-Sorter

And here's my review of the (then) Avalon Cell Sorter:  http://ucflow.blogspot.com/2012/08/propel-labs-avalon-first-look.html
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Bunch of great talks today at the New England Cytometry User Group Meeting.  I was fortunate to be able to share some of the work going on at UCFlow regarding instrument sensitivity testing.  I described our method for calculating the qNORM (quantitative Normalized Overlap Ratio Metric), which is a value that describes the minimum number of antibodies bound to a cell that can be resolved from unstained cells.
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Scott Sneddon's profile photoCarol Oxford's profile photoUCFlow's profile photo
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Forthcoming, Carol.  It's part of a larger publication that I'm waiting for my collaborators to wrap things up.
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Flow Cytometry Core Facility New Year's Resolutions
It's that time of year again when the gyms are packed and weight-loss commercials air continuously.This year, why not turn you attention towards your core facility and come up with some resolutions the whole lab can take part in. The best part is you'll hav...
It's that time of year again when the gyms are packed and weight-loss commercials air continuously.This year, why not turn you attention towards your core facility and come up with some resolutions the whole lab can take part in. The best part is you'll have help from the rest of your lab mates ...
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A First Look at the Beckman Coulter CytoFLEX - Strong Performance in a Small Box
Over the past few years, we've been inundated with small, inexpensive cytometers with the promise that they can perform as well as the big boys. Up until now, I would have told you not to waste your time... up until now. In 2013, an unknown company called X...
Over the past few years, we've been inundated with small, inexpensive cytometers with the promise that they can perform as well as the big boys. Up until now, I would have told you not to waste your time... up until now. In 2013, an unknown company called Xitogen set up a booth at the annual ...
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He said, She said, PEBCAC?
In flow cytometry core facilities, scenarios such as the one that follows are commonplace. The first thought that comes to me after a user reports a problem. An end-user is attempting to collect data, for some reason there's an issue, the end-user requests ...
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If you're even remotely involved in the world of cytometry, you'll want to visit the new community.  Hoping to make this a team effort!
Cytometry
A community dedicated to flow and image cytometry
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Hudson Ansley's profile photoRyan Duggan's profile photo
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That's likely the issue Carol.  You'll need to engage with communities from a desktop for the time being :(

If that doesn't work, you can always just do a search for Cytometry and it'll pop right up.
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Life Technologies Attune Cytometer, take 2
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Ryan Duggan's profile photoScott Sneddon's profile photo
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Well, what I can say is that they've greatly tightened their spec, which is a plus. Also, I think part of the issue revolved around the Applied Biosystems merger. I know for my 1st install, they had our regional sequencer engineer install the attune. I think they are better cross-trained on the flow equipment. And, they've learned their lesson about shoddy installs. I know first hand they lost sales on account of my first review. I'm willing to give them a second chance
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Map of the business location
924 E 57th St r022 Chicago, IL 60637
924 East 57th Street r022USIllinoisChicago60637
(773) 702-5582ucflow.uchicago.edu
LaboratoryToday Closed
Monday 9:00 am – 5:00 pmTuesday 9:00 am – 5:00 pmWednesday 9:00 am – 5:00 pmThursday 9:00 am – 5:00 pmFriday 9:00 am – 5:00 pmSaturday ClosedSunday Closed
The University of Chicago Flow Cytometry Core Facility has been providing flow and image cytometry services for over 20 years.  We have the latest hardware in cytometry technology and years of experience in application development both in the basic research and clinical research fields. We provide service to investigators both inside and outside of the University of Chicago.  If you'd like to collaborate on a project, please contact us.
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