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Zephyr López Cervilla
Attended University of Barcelona
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Zephyr López Cervilla

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adamvstheman.com - Interview to Anthony Antonello on Pro-Gun Control Propaganda Tactics
By Adam Kokesh (host of Adam Vs. the Man). April 15, 2014
youtu.be/8ZqeHUhFYTg (21 min 49 s)

Video blurb:
<< AVTM Producer Anthony Antonello discusses his recent video of OFA organizers describing their propaganda tactics. Not too surprising, but shocking nonetheless. Lots of important info for people who care about gun rights. >>
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Comment: I side with +Mary Mangan's comments on this issue (see below URL of the post that she has re-shared).
I oppose any Government mandate on any issue on moral grounds, GMO labeling is no exception. Consumer demands should be driven by their choices, not Government's use of force and coercion to regulate the market. If some people want to know the exact nature and composition of the product they are going to purchase, its source, and the process applied to produce it, they should choose those products whose labels provide that sort of information. As long as suppliers don't include misleading information (what should be regarded as fraud and treated as a breakage of contract), consumer's freedom of choice is being respected.

URL G+ post reshared by Mary Mangan:
plus.google.com/115686712405727979216/posts/iKpTdJ7AxMY
 
* GMO Labelling Quiz*


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"The dose makes the poison."
– Paracelsus.
en.wikipedia.org/wiki/The_dose_makes_the_poison

Some others will also call poison any drug and psychoactive substance (such as caffeine and pot), or any food containing heavy metals and other industrial contaminants (such as fish, eggs, dairy and meat.)
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freedomainradio.com - Jon Stewart's 19 Tough Questions for Libertarians!
By Stefan Molyneux. October 31, 2011
youtu.be/4hhSsIpjtzY (28 min 14 s)
Comment:
Entertaining and refreshing video from the years in which Stefan hadn't yet acquired his strong pro-wedlock, pro white middle class, pro-psychotherapy, and misogynistic bias. It's funny that he excuses and justifies (in this same video) to those businessmen who play by the rules and lobby the Government to avoid being surpassed by their competition whereas condemning those others who likewise play by the rules and take advantage of the welfare system to try to make their life more comfortable. Either of those bad habits (addictions as he like to call it) encourages poor choices and can lead to terrible consequences.

Those entrepreneurs who use State's power to make their industrial projects grow, are also contributing (perhaps even more) to the perpetuation of a system based on initiation of force and coercion. 
Say, Elon Musk, whose SpaceX contracts with the US Government to supply rockets are further contributing to the consolidation of the system (the satellites used by the intelligence agencies and the armed forces don't appear magically in the sky), and helping maintain the false illusion that the Government is doing something useful with their astronomically expensive space program, or his taking advantage of loans to Tesla Motors to build EVs, and the incentives offered to their costumers to buy their cars. It's even obscene to witness the taxpayer contributing to fund $80,000 for luxury cars to a bunch of wealthy people with enough income to burn such amount in essentially a trendy-looking horse carriage powered by a big washing-machine electric motor.

Answered questions here:
Excerpt of  the original video with some of the questions: thedailyshow.cc.com/videos/wbu22h/andrew-napolitano 
Text: reddit.com/r/austrian_economics/comments/ltrg1/jon_stewarts_19_questions_to_libertarians 

Video blurb/summary:
Stefan Molyneux, host of Freedomain Radio, responds to 19 tough questions for libertarians. Get more from Stefan Molyneux including books, podcasts and other info at: http://www.freedomainradio.com

What would be your best response to these questions and statements:

0:06 - Is government the antithesis of liberty?

0:47 - One of the things that enhances freedoms are roads. Infrastructure enhances freedom. A social safety net enhances freedom.

2:02 - What should we do with the losers that are picked by the free market?

3:38 - Do we live in a society or don't we? Are we a collective? Everybody's success is predicated on the hard work of all of us; nobody gets there on their own. Why should it be that the people who lose are hung out to dry? For a group that doesn't believe in evolution, it's awfully Darwinian.

5:41 - In a representative democracy, we are the government. We have work to do, and we have a business to run, and we have children to raise.. We elect you as our representatives to look after our interests within a democratic system.

7:41 - Is government inherently evil?

9:03 - Sometimes to protect the greater liberty you have to do things like form an army, or gather a group together to build a wall or levy.

9:47 - As soon as you've built an army, you've now said government isn't always inherently evil because we need it to help us sometimes, so now.. it's that old joke: Would you sleep with me for a million dollars? How about a dollar? Who do you think I am? We already decided who you are, now we're just negotiating.

10:54 - You say: government which governs least governments best. But that were the Articles of Confederation. We tried that for 8 years, it didn't work, and went to the Constitution.

11:16 - You give money to the IRS because you think they're gonna hire a bunch of people, that if your house catches on fire, will come there with water.

11:56 - Why is it that libertarians trust a corporation, in certain matters, more than they trust representatives that are accountable to voters? The idea that I would give up my liberty to an insurance company, as opposed to my representative, seems insane.

13:38 - Why is it that with competition, we have such difficulty with our health care system? ...and there are choices within the educational system.

15:00 - Would you go back to 1890?

16:20 - If we didn't have government, we'd all be in hovercrafts, and nobody would have cancer, and broccoli would be ice-cream?

16:30 - Unregulated markets have been tried. The 80's and the 90's were the robber baron age. These regulations didn't come out of an interest in restricting liberty. What they did is came out of an interest in helping those that had been victimized by a system that they couldn't fight back against.

19:04 - Why do you think workers that worked in the mines unionized?

20:13 - Without the government there are no labor unions, because they would be smashed by Pinkerton agencies or people hired, or even sometimes the government.

20:24 - Would the free market have desegregated restaurants in the South, or would the free market have done away with miscegenation, if it had been allowed to? Would Marten Luther King have been less effective than the free market? Those laws sprung up out of a majority sense of, in that time, that blacks should not... The free market there would not have supported integrated lunch counters.

23:23 - Government is necessary but must be held accountable for its decisions.

Credit: reddit.com/r/austrian_economics/comments/ltrg1/jon_stewarts_19_questions_to_libertarians 
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Ron Paul slams US on Crimea crisis and says Russia sanctions are 'an act of war'
By Paul Lewis (The Guardian, Washington). March 15, 2014
theguardian.com/world/2014/mar/15/ron-paul-crimea-russia-sanctions-act-of-war 
Excerpt:
• Paul tells Guardian change in Ukraine is US-backed coup
• Views are opposite to those of son, Senator Rand Paul

<< “The evidence is pretty clear that the NGOs [non-governmental organisations] financed by our government have been agitating with billions of dollars, trying to get that government changed,” he said. “Our hands are not clean.”

There is broad bipartisan support on Capitol Hill for the movement that brought about the departure of Yanukovych, as well as criticism of Putin for Russia’s military intervention in Crimea, >>

<< His son, an increasingly strong contender for the Republican presidential nomination, made a similar point in the Senate on Thursday, when he voted against a bill providing aid to Ukraine.

The Kentucky senator is far more pragmatic than his father, however, >>

<< “His continuing occupation of Ukraine is completely unacceptable, and Russia’s president should be isolated for his actions.”

He added: “Economic sanctions and visa bans should be imposed and enforced without delay.”

His father took the opposite view. “I think sanctions are horrible. They’re acts of war,” he told the Guardian.

“It is based on a moral principle of theft. They want to target sanctions against 20 or 30 bad Russians who they claim have committed a crime against humanity, and therefore we’re going to freeze their assets and steal them from them.”

When it was suggested his position was opposite to that of his son, Paul replied: “Neither he nor I have ever pretended our views are identical. >>

Related column:

• Jonathan Chait. The Pathetic Lives of Putin’s American Dupes. New York magazine. March 14, 2014.
nymag.com/daily/intelligencer/2014/03/pathetic-lives-of-putins-american-dupes.html 

URL related G+ posts: 
plus.google.com/+PradheepShanker/posts/bdzBDhzijrD 
plus.google.com/+EuropeanCommission/posts/QXeXW3TbKFA 
plus.google.com/+presstv/posts/fi19dff2LL7 
plus.google.com/108549873871553806005/posts/VWxqQ7j27bp 
plus.google.com/+EuropeanCommission/posts/AeCAj5kQuQY 
plus.google.com/+EuropeanCommission/posts/CiJbXmFYiL8 
plus.google.com/+BoingBoing/posts/YWK4rtjmMS2 

#ronpaul   #ukraine   #russia   #crimea   #randpaul  
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http://scgnews.com/the-ukraine-crisis-what-youre-not-being-told. Here's a better one, the deception regarding this situation is blatant.
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Zephyr López Cervilla

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plus.google.com - Fight against zealots of time measurement legacy system
Uploaded by 9GAG. March 9, 2014
plus.google.com/+9GAG/posts/frsjechHqKx 
Introduction: They claim that the American system to write measurements of time makes no sense, is "retarded", and that they made the wrong choice whereas "the rest of the world is not wrong" because they use a "consistent" system to list measurements in different time units.

Excerpt from comments of G+ post:

Bruno Bertucci Mar 9, 2014 12:28 AM
Only in the retarded American system with the month before the day
__________ 

Zephyr López Cervilla Mar 9, 2014 12:31 AM
+Bruno Bertucci: "Only in the retarded American system with the month before the day"
— So I guess you'll be able to explain why your system is more clever, right?
__________ 

Daniel Barron Mar 9, 2014 1:38 AM
+Zephyr López Cervilla it's not hard to explain. When listing any measurement the numbers are always listed in left to right with consistent increasing or decreasing size. E.g. Thousands, hundreds, tens, units. MM DD YY makes no sense. YY MM DD or DD MM YY do but ONLY Americans chose the wrong one. The rest of the world is not wrong.
__________ 

Zephyr López Cervilla Mar 9, 2014 1:48 AM
+Daniel Barron: "When listing any measurement the numbers are always listed in left to right with consistent increasing or decreasing size."

— So on which side and order do you list hours, minutes and seconds as in the above example?

+Daniel Barron: "MM DD YY makes no sense. YY MM DD or DD MM YY do"

— Can you show me a newspaper or magazine from anywhere in the world that will list the dates as YY MM DD? The only I'm aware of who use such notation are the astronomers.

Finally, if the rest of the world is so clever, why do they still use hexadecimal [edit: sexagesimal] unit system to divide the hour into minutes and seconds, and why do they divide the day into 24 hours? This doesn't seem very clever to me.
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Robbie Tian Mar 9, 2014 5:07 AM +1
In China we use "yyyy  mm dd" system. 
__________ 

Melane Montejo Mar 9, 2014 8:35 AM
Yes, just like here in the Philippines
__________ 

Daniel Barron Mar 9, 2014 9:40 AM
+Zephyr López Cervilla To answer your questions.  YYYY MM DD hh:mm:ss.  Other people have given examples of that order which answers the second question.  But also I use it when versioning documents.  An example is notes2014/03/09 for a file name.  That way it sorts better on screen.

Regarding the rest of the world being clever.   That's a pointless argument.  We're not talking about that - just the sensible ordering of units.

Hexadecimal is base 16, nothing to do with time measurements.
__________ 

Zephyr López Cervilla Mar 9, 2014 2:14 PM
+Daniel Barron: "Hexadecimal is base 16,"

— I already knew it, to the point that I suggested a series of symbols for a hexadecimal numbering system for its generalised use in several previous G+ posts:

2011.11.06: plus.google.com/+TreyRatcliff/posts/g3aU4CmsK1z 
2011.11.06: plus.google.com/+ZephyrLópezCervilla/posts/gQzkUHaMFvN 
2012.12.06: plus.google.com/110685273879923679231/posts/R46Gc4GUyP3 
2012.12.06: plus.google.com/+ZephyrLópezCervilla/posts/92e6Hi3XN6u 
2014.01.16: plus.google.com/+MattMastracci/posts/MJZp1C2dFp3 
2014.01.16: plus.google.com/+ZephyrLópezCervilla/posts/YBkpa99U2m1 

What I actually meant in the above comment is a sexagesimal unit system
en.wikipedia.org/wiki/Sexagesimal 

Sometimes called "hexagesimal":
en.wikipedia.org/wiki/Hexagesimal 
thefreedictionary.com/hexagesimal 
dictionary.reference.com/browse/hexagesimal 


+Daniel Barron: "nothing to do with time measurements."

— Nope, it does have something to do:
en.wikipedia.org/wiki/Hexadecimal_time 
I guess you meant nothing to do with the most popular system used to express time measurements.


+Daniel Barron: "Other people have given examples of that order which answers the second question."

— While the Chinese list time unit in decreasing order from left to right, e.g., 

<< 2008-09-10 21:55:21 >>
Source: cmse.gov.cn/know/show.php?itemid=23 

<< 4月10日,美国总统奥巴马为NASA提出了177亿美元的2014财年预算申请,比2012财年少了5460万美元,比2013财年少了1.78亿美元。其中国际空间站项目以30.491亿美元成为份额最大的单个项目。>>
Source: cmse.gov.cn/video/show.php?itemid=2595 

Their Chinese Government does use the American notation to translate dates from Chinese into English in their own websites. E.g.,

Shenzhou I
Launch time: At 6:30:07 on November 20, 1999
Source: en.cmse.gov.cn/list.php?catid=36 

As for the Philippines, the following are excerpts from the official website of the Government of the Philippines:

<< Appointments and Designations: February 19, 2014 >>
Source: gov.ph/2014/02/19/appointments-and-designations-february-19-2014 

<< Message of President Aquino to the Visayan Village Tagum Rizal Works and Multi-Purpose Cooperative on the occasion of their 4th General Assembly, March 9, 2014 >>
Source: gov.ph/2014/03/09/message-of-president-aquino-to-the-visayan-village-tagum-rizal-works-and-multi-purpose-cooperative-on-the-occasion-of-their-4th-general-assembly-march-9-2014 

<< Statement of Secretary Edwin Lacierda:
On the week of March 3-7, 2014
[Released on March 3, 2014] >>
Source: gov.ph/2014/03/03/statement-the-presidential-spokesperson-on-the-week-of-march-3-7-2014 

<< Inflation eases in February 2014 >>
Source: gov.ph/2014/03/05/inflation-eases-in-february-2014 


— So I would call it BS.

Likewise, the journal Nature published by an British publisher and using British English conventions, lists dates as DD-MM-YYYY ratherthan the other way around (YYYY-MM-DD):

<< 06 March 2014 >>
nature.com/news/head-of-china-s-space-science-reaches-out-1.14797 

<< ranked the world's most cited by the Science Edition of the 2010 Journal Citation Reports and is widely regarded as one of the few remaining academic journals that publish original research across a wide range of scientific fields.[2] >>
Source: en.wikipedia.org/wiki/Nature_(journal) 

As well as in the website of the Royal Society:

<< 07 March 2014 >>
Source: royalsociety.org/news/2014/diversity-data-report 

And in  the Royal Society's own journals:

_<< Published 24 February 2014 doi: 10.1098/rsta.2013.0105
Phil. Trans. R. Soc. A 28 March 2014 vol. 372 no. 2012 20130105 >>_
Source: rsta.royalsocietypublishing.org/content/372/2012/20130105.abstract 

<< Research article: Analog synthetic biology
Phil. Trans. R. Soc. A. 2014 372 20130110; doi:10.1098/rsta.2013.0110 (published 24 February 2014) >>
Source: rsta.royalsocietypublishing.org/content/372/2012.toc 


<< The Royal Society of London for Improving Natural Knowledge, commonly known as the Royal Society, is a learned society for science, and is possibly the oldest such society still in existence.[1] Founded in November 1660, it was granted a Royal Charter by King Charles II as the "Royal Society of London". The Society today acts as a scientific advisor to the British government, receiving a parliamentary grant-in-aid. The Society acts as the UK's Academy of Sciences, and funds research fellowships and scientific start-up companies. >>
en.wikipedia.org/wiki/Royal_Society 


+Daniel Barron: "Other people have given examples of that order which answers the second question.  But also I use it when versioning documents.  An example is notes2014/03/09 for a file name.  That way it sorts better on screen."

— I  had a look at your posts and guess what, I found the following file names of pictures uploaded by you:

<< File name        29/08/2013 - 1 >>
<< Camera        iPhone 5 >>
Source: plus.google.com/+DanielBarron/posts/NvohXzKocN1 

<< File name        01/08/2013 - 1 >>
<< Camera        iPhone 5 >>
Source: plus.google.com/+DanielBarron/posts/i3CsY3t1K7x 

<< File name        11/07/2013 - 1 >>
<< Camera        iPhone 5 >>
Source: plus.google.com/+DanielBarron/posts/LBniK93WCXN 

<< File name        13/06/2013 - 1 >>
<< Camera        iPhone 5 >>
Source: plus.google.com/+DanielBarron/posts/EGap4uAEAoX 

<< File name        13/06/2012 - 1 >>
<< Camera        iPhone 4S >>
Source: plus.google.com/+DanielBarron/posts/ar2xu3u3rpv 

<< File name        01/11/2011 >>
<< Camera        - >>
Pic: 
lh3.googleusercontent.com/-HdxDynkw8sc/TrBT1HIQblI/AAAAAAAAEUk/--VF2A6dWOU/s640-no/2011 
Source: plus.google.com/photos/of/112471430236137314656 


I also noticed that DD/MM/YYYY wasn't the default file naming in your iPhone 5S, iPhone 5, or Samsung:

<< File name        IMG_8461.JPG >>
<< Camera        iPhone 5s >>
Source: plus.google.com/+DanielBarron/posts/dX1gF57iMyX 

<< File name        IMG_9550.JPG >>
<< Camera        iPhone 5 >>
Source: plus.google.com/+DanielBarron/posts/Lram8LBtr8u 

<< File name        SDC15686.JPG >>
<< Camera        SAMSUNG ES55,ES57 / VLUU ES55 / SAMSUNG SL102 >>
Pic: lh4.googleusercontent.com/-iyBfrDC82xk/URRLx6l1-_I/AAAAAAAAGLw/XynPQIn_vv8/w2238-h1259-no/SDC15686.JPG 
Source: plus.google.com/photos/of/112471430236137314656 


— So it seems that you haven't been lately practicing what you preach.


+Daniel Barron: "Regarding the rest of the world being clever.   That's a pointless argument.  We're not talking about that"

— No it isn't pointless. Read again the comment that I posted to which you first replied:

+Bruno Bertucci: "Only in the retarded American system with the month before the day"
— So I guess you'll be able to explain why your system is more clever, right?

I asked Bruno Bertucci to provide an explanation on why he thinks that the American system (MM-DD) is "retarded" compared to the other systems (that is, any other with the day before the month, DD-MM). Interestingly, he excluded implicitly YYYY-MM-DD since, as in the case of the American system, YYYY-MM-DD also has also the month before the day.

And now read again your reply:

+Daniel Barron: "MM DD YY makes no sense. YY MM DD or DD MM YY do but ONLY Americans chose the wrong one. The rest of the world is not wrong."

— The rest of the world (except for the Chinese when they write in Chinese but not when they translate into English) including the British academic world majorly use DD-MM-YYYY. However, such system is inconsistent with the system that the rest of the world use to list hours, minutes, and seconds, hh:mm:ss.

Finally, you haven't answered my question of why you still use a combination of a decimal system, a duodecimal system (base-12), a 28/29-30-31-base variable system, a quadrivigesimal system (base-24) and a sexagesimal system (base-60) if it's comparatively much more cumbersome than a time unit system in the same base as your usual numbering system.
__________ 

URL source G+ post: 
plus.google.com/+9GAG/posts/frsjechHqKx 

Further reading: 
oeis.org/wiki/Sexagesimal_numeral_system 
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scientificamerican.com - The Moral Life of Babies
By Gareth Cook (Scientific American). November 12, 2013 
scientificamerican.com/article/the-moral-life-of-babies 
Yale Psychology Professor Paul Bloom finds the origins of morality in infants

Excerpt:
<<
What are the first signs of morality in babies?
The earliest signs are the glimmerings of empathy and compassion—pain at the pain of others, which you can see pretty soon after birth. Once they’re capable of coordinated movement, babies will often try to soothe others who are suffering, by patting and stroking. >>

Comment:
Unlike Paul Bloom statements, I consider morality exclusively as a social construct. He equates morality with empathy (and fairness), two quite distinct things that can easily coexist independently. 

For instance, mothers of criminals quite often feel empathy toward their sons despite recognising their actions as immoral. Their empathy and moral judgement shed conflicting results because they are in fact different decision making mechanisms. In such situations empathy tends to prevail over morality.

In my view, morality is a set of rules aimed to facilitate coexistence and avoid conflicts within a particular community, generally based on balance of might, that are generally accepted by the members of a particular community, and that people tend to learn and internalise to judge the actions of other members and their own.

This also helps explain why moral principles differ from one community to another, and from one period of history to other periods. In contrast, empathy is a more stable and linked to some genetic component, as Paul Bloom points out. 

Quite often morality will override empathy, this explains why many people may demand punitive actions (physical and psychological punishment beyond any protective measure) against individuals who they judge immoral, confronting any feeling of empathy that they may keep towards them.

This also explain why the examiner who acts based on moral principles will evaluate their examinees independently from the empathy they may feel toward them.

URL source G+ post: 
plus.google.com/+MehranSharmini/posts/hSJMKTE6Y3k 
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Zephyr López Cervilla

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Stefan, you should be ashamed to spread (again) disinformation: that the single worst predictor of being a criminal (a rapist, or whoever other wrongdoer) is being a child reared by a single mum, and that a 10% of the children aren't the biological offspring of the men who have recognised them as their legal fathers and believe they are their biological fathers. In this latter subject I proved you wrong in another video, so I won't post my evidence once again. Anyway, I challenge you to post your references or otherwise retract what you've stated.
________ 

<< Disinformation is intentionally false or inaccurate information that is spread deliberately. It is an act of deception and false statements to convince someone of untruth. Disinformation should not be confused with misinformation, information that is unintentionally false.

Unlike traditional propaganda techniques designed to engage emotional support, disinformation is designed to manipulate the audience at the rational level by either discrediting conflicting information or supporting false conclusions. A common disinformation tactic is to mix some truth and observation with false conclusions and lies, or to reveal part of the truth while presenting it as the whole (a limited hangout). >>
en.wikipedia.org/wiki/Disinformation 
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Registering a child isn't an admission of biological paternity by the father, to the point that either the mother or the father can request a paternity test at any time. Registering as the father of a child is equivalent to adopting it, no biological link is required. Otherwise, a mandatory paternity test would be required to be accepted.

There are numerous examples of men who have recognised as their child newborn babies who couldn't possible to be their own children. For instance, there used to be the common practice among single men to marry their brother's widow and adopt her children. This would include any not yet born child. These kids weren't their own and yet, they appear in the records as their father.
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adamvstheman.com* - Interview to Jaric Fontaine, Democratic Candidate for Maine Senate District 10*
By Adam Kokesh (Adam Vs. the Man). April 13, 2014
youtu.be/IyCAIQl5zx4 (31 min 35 s)

Video blurb:
<< Adam debates a statist from Maine on all things economical. Hilarity ensues, but not everyone is laughing! Thank you, Jaric Fontaine, for joining us. >>

bangordailynews.com/community/meet-your-democratic-candidates-jaric-fontaine-and-mary-sharkus
________

Excerpt:
"From 1940s to 1980s we had one of the most stable growth rates in history. Wages were on line with productivity, we had the strongest middle class, we had the best scientists, we had the best jobs, and we had the most stable banking system in history. We didn't have one failure until the deregulation of the banks starting in the 80 with Reagan," [8:13]

Comment:
There wasn't price stability during the 1970s. There was strong inflation coupled to an economic recession:
en.wikipedia.org/wiki/Nixon_Shock
en.wikipedia.org/wiki/1973–75_recession

As for his defence of inflation ( "I want prices to continue to rise in line with wages." [6:11]), or of an artificial price stability while the cost of production goes down, I would ask him whether he cares for the environment and whether he thinks that it's a good thing for the environment to encourage people to burn their money and consume today because their money is going to be valued less tomorrow.

On the other hand, deflation wasn't the cause of the Great Depression but rather one of its consequences. As demand went down supply became cheaper.
_________________

URL source G+ post:
plus.google.com/101655429342193303969/posts/5vZydShd3j6
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Zephyr López Cervilla

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Void/Dead Volume (V sub o / V sub M)

1. Malik A and Jinno K. Determination of void/dead volume of liquid chromatographic columns containing β-cyclodextrin as the stationary phase. Part I. Existing methods for the determination of LC column dead volume—A survey of literature. Chromatographia (1990) vol. 30 (3-4) pp. 135-137
URL: link.springer.com/article/10.1007/BF02274534 

Comment: Behind paywall, but still the two pages with all the content of this short paper are freely available, only most of the references aren't freely available.

2. González Pérez, Claudio. Cromatografía de exclusión molecular. (pages 10 to 14). 16101 - Análisis aplicado a la Ingeniería Química, 2008-09. Tema 12. Cromatografía Líquida. Departamento Química Analítica, Nutrición y Bromatología. Universidad de Salamanca (2008) pp. 2-49
URL (PDF): ocw.usal.es/ciencias-experimentales/analisis-aplicado-a-la-ingenieria-quimica/contenidos/course_files/Tema_12.pdf 

Question: Is it common to refer to the volume of the cannula through which empties the column as the dead volume instead of considering dead volume and void volume as the same thing?

Related links:

"Molecular Weight Ranges for Gel Filtration Media"

Gel Filtration Chromatography. Sigma-Aldrich
PDF: sigmaaldrich.com/content/dam/sigma-aldrich/life-science/proteomics-and-protein/proteomics/chromatography_gel_filtration.pdf 

Size Exclusion Chromatography (Gel Filtration Chromatography)
chemistry.armstrong.edu/nivens/chem3801/SizeExclusionChromatography.htm 

• Amersham Biosciences. Gel Filtration: Principles and Methods. Handbooks rom Amersham Biosciences (2002)
PDF: kirschner.med.harvard.edu/files/protocols/GE_gelfiltration.pdf 

• GE Healthcare Bio-Sciences. Gel Filtration: Principles and Methods. Handbooks from GE Healthcare (2002)
PDF: sevierlab.vet.cornell.edu/resources/Gel_Filtration_Book.pdf 

Guide to Gel Filtration or Size Exclusion Chromatography. Harvard Apparatus
PDF: harvardapparatus.com/guide+for+gel+filtration.pdf 

• Thompson ST et al. Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins. Proc Natl Acad Sci USA (1975) vol. 72 (2) pp. 669-72
pnas.org/content/72/2/669 
ncbi.nlm.nih.gov/pmc/articles/PMC432376 
(ncbi.nlm.nih.gov/pubmed/164664)

• Ming-Che L. Chapter 5 : Other Separation Techniques. Plant Developmental and Molecular Biology Laboratory. National Chung Hsing University (2012)
PDF: nchu.edu.tw/~giab/giabcsw/molecular%20biotechology/chapter%205.pdf 
Parent directory: life.nthu.edu.tw/~yjsun/main 

• *Biotechnology Explorer Size Exclusion Chromatography
Instruction Manual.* Bio-Rad
PDF: tccbiotech.org/files/documents/Chromatography_BioRad_Manual__teachers_manual_includes_student_manual_.pdf 

Size exclusion chromatography. Wikipedia in English.
en.wikipedia.org/wiki/Size-exclusion_chromatography 

Sephadex® G-25 media and pre-packed columns. Amersham Biosciences
PDF: gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314716762536/litdoc18111579AB_20110830184813.pdf 

Sephadex® G-25 media and pre-packed columns. Pharmacia Biotech
PDF: chembio.uoguelph.ca/educmat/chm357/g25.pdf 

• Beckman Coulter. JA-20 Fixed Angle Rotor. Beckman Coulter Inc. (2008)
beckmancoulter.com/wsrportal/techdocs?docname=J-TB-003 
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Zephyr López Cervilla

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Swine study suggests flu vaccination may sometimes backfire
By Lisa Schnirring (CIDRAP News). August 29, 2013
cidrap.umn.edu/news-perspective/2013/08/swine-study-suggests-flu-vaccination-may-sometimes-backfire 

Excerpt:

<< During the 2009 H1N1 pandemic, Canadian researchers identified a greater risk of infection in those who had been vaccinated against seasonal flu >>

<< studies in the United States and other countries contradicted the Canadian findings. However, in 2010 a large study by a Canadian team put an exclamation point on the earlier findings, reporting that the risk of needing treatment for pandemic flu was 1.4 to 2.4 times greater in those who had been vaccinated against seasonal flu in the previous year.

In the new study, researchers explored the phenomenon in pigs that were infected with the 2009 H1N1 virus after receiving a vaccine against H1N2 influenza. The team, headed by scientists from the US Food and Drug Administration (FDA) and the US Department of Agriculture (USDA), published its findings yesterday >>

<< The interaction between the vaccine and respiratory disease, called vaccine-associated enhanced respiratory disease (VAERD), has been seen before, for example with formaldehyde-inactivated respiratory syncytial virus (RSV) vaccination followed by wild-type RSV infection.

The researchers vaccinated piglets that were younger than 6 months old and hadn't been exposed to flu before against H1N2 to explore whether vaccine-induced antibodies might play a role in exacerbating respiratory symptoms. They found some of the piglets got sick with severe pneumonia and had severe lung damage after they were infected with the 2009 H1N1 virus, suggesting that cross-reactive antibodies triggered by the flu vaccine made symptoms worse after infection with a different flu strain.

The part of the experiments that focused on the mechanism responsible for the effect found that H1N2 antibodies in the sick pigs bound to the hemagglutinin (HA) stem rather than the HA head of the 2009 H1N1 virus, and thus weren't able to block the virus from attaching to cells. Instead, the strong cross-reactive antibodies helped the pandemic virus fuse to cell membranes, which appeared to worsen respiratory problems in the vaccinated pigs. >>


Articles and blog posts related to flu vaccine's efficacy and adverse effects:

• Vincent Racaniello. Attenuated influenza vaccine enhances bacterial colonization of mice. Virology Blog. March 12, 2014.
virology.ws/2014/03/12/attenuated-influenza-vaccine-enhances-bacterial-colonization-of-mice 

Reference paper: 
• Mina MJ et al. Live Attenuated Influenza Vaccine Enhances Colonization of Streptococcus pneumoniae and Staphylococcus aureus in Mice. MBio (2014) vol. 5 (1) doi:10.1128/mBio.01040-13
mbio.asm.org/content/5/1/e01040-13.full 
(ncbi.nlm.nih.gov/pubmed/24549845)

• Vincent Racaniello et al. TWiV 217: I just flu in and my arms are shot. This Week in Virology. January 27, 2013.
twiv.tv/2013/01/27/twiv-217-i-just-flu-in-and-my-arms-are-shot 

• Vincent Racaniello. Effectiveness of this season’s influenza vaccine. Virology Blog. January 18, 2013.
virology.ws/2013/01/18/effectiveness-of-this-seasons-influenza-vaccine 

• Vincent Racaniello. Friday flu shot. Virology Blog. January 11, 2013.
virology.ws/2013/01/11/friday-flu-shot-2 

• Vincent Racaniello. How good is the influenza vaccine? Virology Blog. November 3, 2011.
virology.ws/2011/11/03/how-good-is-the-influenza-vaccine 

• Katherine Hobson. Flu Shot Could Be Better, But For Now It’s The Best We’ve Got. The Wall Street Journal (blogs). October 26, 2011.
blogs.wsj.com/health/2011/10/26/flu-shot-could-be-better-but-for-now-its-the-best-weve-got 

Reference paper: 
• Osterholm MT et al. Efficacy and effectiveness of influenza vaccines: a systematic review and meta-analysis. The Lancet Infectious Diseases (2012) vol. 12 (1) pp. 36-44
(ncbi.nlm.nih.gov/pubmed/22032844)

Press release:
Gaps in evidence for effectiveness of influenza vaccines highlight need for new vaccine. Eurekalert. October 25, 2011.
eurekalert.org/pub_releases/2011-10/l-gie102411.php 

Reference report:
• Osterholm MT et al. The Compelling Need for Game-Changing Influenza Vaccines. An Analysis of the Influenza Vaccine Enterprise and Recommendations for the Future. University of Minnesota Center for Infectious Disease Research and Policy (2012)
cidrap.umn.edu/cidrap/center/mission/articles/ccivi-landing.html 

Video interview to leading author:
• Michael T. Osterholm (interviewee). Influenza. UPMC Center for Health Security. April 25, 2013.
cidrap.umn.edu/compelling-need-game-changing-influenza-vaccines 
youtu.be/pt1CLKeWWZM (8 min 50 s)

• Vincent Racaniello (interviewer) and Frederick Hayden (interviewee). Frederick Hayden on influenza antivirals. [part of TWiV 99: TWiV at ICAAC 2010] Virology Blog. January 31, 2011.
virology.ws/2011/01/31/frederick-hayden-on-influenza-antivirals 
youtu.be/qpk-SVzx23I (15 min 29 s)


Specialised press:

• Maroof A et al. Intranasal vaccination promotes detrimental Th17-mediated immunity against influenza infection. PLoS Pathog (2014) vol. 10 (1) pp. e1003875
plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1003875 
(ncbi.nlm.nih.gov/pubmed/24465206)

• Aspinall R and Lang PO. The Avalanche is Coming … And Just Now It's Starting to Snow. Front Immunol (2013) vol. 4 pp. 165
journal.frontiersin.org/Journal/10.3389/fimmu.2013.00165/full 
(ncbi.nlm.nih.gov/pubmed/23805142)

• Lenzer J. Belief not science is behind flu jab promotion, new report says. BMJ (2012) vol. 345 pp. e7856
bmj.com/content/345/bmj.e7856 
(ncbi.nlm.nih.gov/pubmed/23166084)

• Flegel K. Health care workers must protect patients from influenza by taking the annual vaccine. CMAJ (2012) vol. 184 (17) pp. 1873
cmaj.ca/content/184/17/1873.long 
(ncbi.nlm.nih.gov/pubmed/23109612)

• Flegel K. Influenza vaccination of health care workers--the author responds. CMAJ (2013) vol. 185 (2) pp. 151
stm.sciencemag.org/content/5/200/200ra114.abstract 
(ncbi.nlm.nih.gov/pubmed/23382376)

• Doshi P et al. Influenza vaccination of health care workers. CMAJ (2013) vol. 185 (2) pp. 150
cmaj.ca/content/185/2/150.1.long 
(ncbi.nlm.nih.gov/pubmed/23382374)

• Doshi MT et al. Influenza vaccination of health care workers. CMAJ (2013) vol. 185 (2) pp. 150-1
cmaj.ca/content/185/2/150.2.long 
(ncbi.nlm.nih.gov/pubmed/23382375)


Comment: the following letter is clearly biased against but it contains references to some informative studies, articles and posts. I've already included some of them in this post.

• Eisenstein M MD,JD,MPH Scientific Evidence about the efficacy and safety of flu vaccines are universal mandates on the way?
library.constantcontact.com/download/get/file/1101240076659-1471/EFFICACY+AND+SAFETY+OF+FLU+VACCINES.pdf 


Excerpt from comments of related G+ post:

Zephyr López Cervilla Mar 15, 2014 4:39 AM
I'm not vaccinated for measles either. I have personally nothing against vaccines. In fact, some years ago I visited 3 times a healthcare center to get 3 shots of the Hepatitis A and B vaccine. But now I don't feel like to get the MMR vaccine, I simply avoid being exposed to people suspicious to carry a contagious disease, and not weakening my immune system (e.g., by sleep deprivation, chill, zinc, or vitamin D deficiency).

When I was a kid my family didn't want me to take the MMR shot, I don't know the exact reason, they vaccinated me of other diseases such as polio. Perhaps the MMR didn't have a good reputation back then, or perhaps they considered the MMR to be rather useless. But this is just my lucubration.

BTW, I'm certain that a few of my classmates back then or their siblings got infected with measles or mumps, and quite probably they had been vaccinated (possibly I was the only who hadn't been vaccinated in my class), so as someone said above, the measles vaccine isn't very effective either.

A few years ago I recommended my old mother to get a shot of the seasonal flu vaccine. The resulting effects of the immunisation made her feel ill for a few days. She hasn't gone to have another shot since then (her own initiative). Anyway, she's never passed the flu, or she's never experienced its symptoms, so why worry? If they ever commercialise an effective and long-lasting flu vaccine perhaps I'll recommend it to her.


Suzanne Roberts Mar 15, 2014 5:47 AM +1
+Zephyr López Cervilla two things - herd immunity and carriers. 
Herd immunity is what protects us (no vaccine can be 100% effective, but the more people in the community that ARE vaccinated, the less chance of spreading the disease. 
Carriers - persons who have been exposed to a virus do not always exhibit symptoms...do you want to risk infecting a child, an elderly person, someone who is immuno-deficient? I certainly don't.

In your mother's case, it is certainly possible that the vaccine was for a different mutation of influenza, or that her symptoms were caused by something other than influenza, like a norovirus.

People seem to forget that influenza (and other vaccine-preventable diseases) caused hundreds of thousands of deaths worldwide.


Zephyr López Cervilla Mar 15, 2014 8:31 AM
+Suzanne Roberts: "two things - herd immunity and carriers."

Two things too. First, you aren't telling me anything I haven't heard before. I took a couple of courses of immunology while in college (in addition to the lessons of immunology in human physiology and in some other subjects). 

Second, for some reason you have assumed that I care enough the health of anonymous others to the point I'll get shots that will probably never help me. 

As for my mother, why should she bother to get any flu vaccines? they won't make her feel any better but rather the other way around. She must have been already exposed to influenza viruses in hundreds of occasions in the past, if hasn't passed the flu so far she won't probably get the disease unless her health deteriorates significantly, but at that point, all her previous years' flu shots won't make her any good either.


Edit: it seems the year after that flu shot she caught a cold particularly bad, something unusual to her and that she attributes to the vaccine. It may well be that that was actually a flu and not a cold, and that her atribution to stronger symptoms to her vaccination isn't actually far fetched:

• Lisa Schnirring. Swine study suggests flu vaccination may sometimes backfire. CIDRAP News. Aug 29, 2013.
cidrap.umn.edu/news-perspective/2013/08/swine-study-suggests-flu-vaccination-may-sometimes-backfire 


On the other hand, I can't feel the difference between a flu or a cold either, so I have no intention to take any flu shots either. And as for the peace of mind of those who are public health-conscious, fuck them all.

If you really want to cut down the spread of contagious diseases then you should advocate against herding kids in daycare centres and schools. Those centres work as bioreactors, allowing to grow and recombine different strains of contagious diseases that then they will spread to their families (including the elderly great-grandpas and grandmas).

I don't mean that children should never socialise with other kids, the problem arises when you put hundreds or even a thousand kids in the same building or concentration camp for 6-8 hours a day 9 months a year. Considering how scrupulous children are with their personal hygiene it'd take a miracle that they didn't propagate to others any contagious disease, fleas, lice, worms, or whatever else they may have caught.

+Suzanne Roberts: "do you want to risk infecting a child, an elderly person, someone who is immuno-deficient? I certainly don't."

— I am certainly more concerned of the risk of becoming infected from others. Contagious diseases usually come from someone else.
If nobody infects me I won't infect an elderly person. I try to avoid contact with children (as I said above children are primary carriers for contagious diseases) so I have hardly any chance to infect a child.

+Suzanne Roberts: "it is certainly possible that the vaccine was for a different mutation of influenza, or that her symptoms were caused by something other than influenza, like a norovirus."

— You seem not to have got it. She didn't pass the flu, those were the typical symptoms caused by the effects of the vaccination. The real flu also causes other symptoms like cough, runny nose and lasts longer than two days. As for noravirus, that usually causes gastroenteritis, and those weren't her symptoms. In addition, the timing was perfect, and she rarely feel ill. If you want I can find you to a recording with the description of the symptoms experienced after a flu shot by a reputed virologist.

+Suzanne Roberts: "People seem to forget that influenza (and other vaccine-preventable diseases) caused hundreds of thousands of deaths worldwide."

— As I said above, I don't are much about that. People are going to die one way or another, if not by disease, by wars or by their own stupidity. Granted, they are entitled to take care of themselves and their health but don't ask me to make sacrifices to help others that I don't even know. The only reason I would consider to get a vaccine is to protect myself, that's why I got the hepatitis vaccines.
___________ 

URL source G+ post: 
plus.google.com/+BetsyMcCall/posts/FX1zy7SfiY4 
________________________ 
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Zephyr López Cervilla's profile photo
 
There's misinformation on both sides. I'm sick of the trend of using false claims and manipulate facts in order to further political and/or ideological agendas:

"Only the nasal mist vaccine contains a live virus, which is severely weakened and harmless."

• Maroof A et al. Intranasal vaccination promotes detrimental Th17-mediated immunity against influenza infection. PLoS Pathog (2014) vol. 10 (1) pp. e1003875
plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1003875
(ncbi.nlm.nih.gov/pubmed/24465206)

• Vincent Racaniello. Attenuated influenza vaccine enhances bacterial colonization of mice. Virology Blog. March 12, 2014.
virology.ws/2014/03/12/attenuated-influenza-vaccine-enhances-bacterial-colonization-of-mice 

Reference paper: 
• Mina MJ et al. Live Attenuated Influenza Vaccine Enhances Colonization of Streptococcus pneumoniae and Staphylococcus aureus in Mice. MBio (2014) vol. 5 (1) doi:10.1128/mBio.01040-13
mbio.asm.org/content/5/1/e01040-13.full 
(ncbi.nlm.nih.gov/pubmed/24549845)


"Maher has said even crazier anti-vax stuff, and has promoted the debunked "vaccines cause autism" rumor,"

— It wasn't just a rumor. Regardless of how inaccurate it was, there was a report published in a reputed medical journal (The Lancet) suggesting such thing:

• Wakefield AJ et al. Ileal-lymphoid-nodular hyperplasia, non-specific colitis, and pervasive developmental disorder in children. The Lancet (1998) vol. 351 (9103) pp. 637-641 [28 February 1998]
briandeer.com/mmr/lancet-paper.htm 
PDF: briandeer.com/mmr/lancet-paper.pdf 

Now you won't find it there because it was pulled out by the publisher.


"he did not believe that healthy people were vulnerable to dying from the new H1N1 virus. This contradicts statements from the World Health Organization and the Centers for Disease Control and Prevention that young, healthy people from ages 5 to 24 appear particularly vulnerable to this flu. About a third of the 76 children who have died of H1N1 since April have had no underlying health problems."

"Lies, damned lies, and statistics."
york.ac.uk/depts/maths/histstat/lies.htm 
en.wikiquote.org/wiki/Leonard_H._Courtney 
en.wikipedia.org/wiki/Lies,_damned_lies,_and_statistics 


"as well as peddling ridiculous anti-gmo false claims,"

— Quite a vague accusation, but not without strong qualifiers.


"he sounds like a kooky survivalist in Idaho or something with his fear of the government injecting him."

— So perhaps the US Government has never performed experiments with people or has performed a medical or surgical intervention on an individual without their consent? :

en.wikipedia.org/wiki/Unethical_human_experimentation_in_the_United_States 


More about the commercial flu vaccines:

<< An independent meta-analysis of vaccines against influenza has found that claims of benefit have been significantly exaggerated.

The report, released last month by the University of Minnesota’s Center for Infectious Disease Research and Policy, was based on a comprehensive review of data published from 1967 to 2012.1

Evidence for “consistent high-level protection is elusive,” the researchers concluded. Although vaccination was found to provide modest protection from infection in young healthy adults who rarely have complications of flu, the authors found that “evidence for protection in adults 65 years of age and older [who represent over 90% of deaths from flu] . . . is lacking.”

The authors also analyzed recommendations issued by the federal Advisory Committee on Immunization Practices, which provides expert advice to the US Centers for Disease Control and Prevention and which are “often considered the standard of practice . . . around the globe.” The report cited 30 instances in which its advisory statements “did not apply current standards of scientific rigor . . . and did not cite relevant work.”

The report’s lead author, Michael Osterholm, a former CDC consultant and an internationally recognized expert on flu, told the BMJ that a Dutch study cited by the CDC as evidence of vaccine efficacy was seriously flawed and constituted a “sales job.” Nevertheless, Osterholm said, the current jab does offer some protection and should be used until a more effective vaccine can be developed.

Joseph Bresee, chief of the epidemiology and prevention branch in CDC’s Influenza Division, told the BMJ, “I do not agree that CDC has inflated the benefits of influenza vaccine.” He added that he agreed with Osterholm that until better vaccines were available the current ones should be recommended.

That recommendation, however, has come under fire from the authors of a Cochrane analysis that also found little to no benefit from flu vaccination.2 Tom Jefferson, lead author of several Cochrane reviews, told the BMJ, “Based on more than a decade of Cochrane reviews in adults, children, [the] elderly, and healthcare workers, there is no credible evidence that the inactivated vaccines have any effect other than saving on average half a working day in healthy adults and avoiding symptoms in those who least need it: healthy adults and adolescents. Depending on the season, you need to immunize 33 to 99 adults to avoid one set of symptoms.”

Osterholm criticized the methods of the Cochrane analysis, saying that the reviewers’ inclusion of studies that used serology titers rather than reverse transcription polymerase chain reaction or cultures to diagnose flu meant that its results were highly inaccurate. However, he acknowledged that the direction of bias caused by use of serology titers would be to make a vaccine seem far more effective than it was, a surprising bias for a meta-analysis that found no benefit for flu vaccines. The Cochrane reviewers also stated in their meta-analysis that the studies reviewed were “at high risk of bias.”

A growing number of healthcare facilities and states now require healthcare workers to be vaccinated against flu or face being fired or forced to wear masks and identification tags stating that they may be infectious.

A recent editorial in the journal of the Canadian Medical Association, CMAJ, recommending mandatory flu jabs for healthcare staff triggered strong criticism, including a letter to the editor from Peter Doshi, a postdoctoral fellow at Johns Hopkins University, and several Cochrane reviewers, who said that the recommendation was based on a misrepresentation of Cochrane data.3 4

Doshi opposes compulsory vaccination. He told the BMJ that health officials “risk losing credibility by continuing to promote the fiction that mandatory influenza vaccination policies are based on solid evidence. They are not, and it is time for healthcare institutions to do their own rigorous assessment of the evidence rather than continuing this dangerous game of follow the leader.”

Osterholm agreed that scientific evidence, not professional opinions, should guide policy. He told the BMJ, “I don’t think the data warrant mandated vaccine. If it was up to me, there are a hundred other things I’d mandate first, like mandating that sick healthcare workers don’t come to work. That is far more likely to be effective.” >>

— Lenzer J. Belief not science is behind flu jab promotion, new report says. BMJ (2012) vol. 345 pp. e7856
bmj.com/content/345/bmj.e7856 
(ncbi.nlm.nih.gov/pubmed/23166084)

Comments on:

• Vincent Racaniello. How good is the influenza vaccine? Virology Blog. November 3, 2011.
virology.ws/2011/11/03/how-good-is-the-influenza-vaccine 

Reference paper: 

• Osterholm MT et al. Efficacy and effectiveness of influenza vaccines: a systematic review and meta-analysis. The Lancet Infectious Diseases (2012) vol. 12 (1) pp. 36-44
(ncbi.nlm.nih.gov/pubmed/22032844)

Reference report:

• Osterholm MT et al. The Compelling Need for Game-Changing Influenza Vaccines. An Analysis of the Influenza Vaccine Enterprise and Recommendations for the Future. University of Minnesota Center for Infectious Disease Research and Policy (2012)
cidrap.umn.edu/cidrap/center/mission/articles/ccivi-landing.html 

Press release:
Gaps in evidence for effectiveness of influenza vaccines highlight need for new vaccine. Eurekalert. October 25, 2011.
eurekalert.org/pub_releases/2011-10/l-gie102411.php 

Video interview to leading author:

• Michael T. Osterholm (interviewee). Influenza. UPMC Center for Health Security. April 25, 2013.
cidrap.umn.edu/compelling-need-game-changing-influenza-vaccines 
youtu.be/pt1CLKeWWZM (8 min 50 s)
__________ 

URL comments related G+ post: 
plus.google.com/+TomEigelsbach/posts/A2vcXtg1Rd6 

en.wikipedia.org/wiki/Vaccine_controversies 
en.wikipedia.org/wiki/Thiomersal_controversy 

stm.sciencemag.org/content/5/200/200ra114 
stm.sciencemag.org/content/5/200/200fs34 
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    Licentiate Biology (Biomedical), 2004 - 2009
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