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Timothy O'Flynn
Works at University College Cork - National University of Ireland, Cork
Attends University College Cork - National University of Ireland, Cork
Lives in Ireland, Cork
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Timothy O'Flynn

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why use two plasmids. Why not out GOI & Transposase into the one plasmid? Saves you twice the amount of transformation no?
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Timothy O'Flynn's profile photoJack Ho's profile photo
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Jack Ho
 
yep definitely
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Timothy O'Flynn

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Timothy O'Flynn

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how does the exonuclease at 1:31 know to keep digesting DNA strand until it comes to the error? Why does it not stop before the error, or not continue on for many more bases after the error?
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Samuel Hardy's profile photoTimothy O'Flynn's profile photoM-L S's profile photo
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M-L S
 
MutS was bound to the mismatch site. The Mut complex just disappeared in the illustration to allow you to see that nuclease removes the nucleotides from the non methylated strand (from the site which was bound to MutH) to the mismatch site (bound to MutS). MutH made a cut in the strand, exonucleases cut nucleic acid from free ends, therefore it was able to recognise the cut and continue from there in the direction of the Muts. Nucleases have specific directions in which they move. A strand is usually synthesised in 5'->3' direction.  Some nucleases can move in both directions, but since the exonuclease mentioned is not specified, you don't know its specifics :) Hope this helps...and I hope I got it all correct :)
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Timothy O'Flynn

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How I feel when I turn the exam paper over to find the whole format has changed
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Timothy O'Flynn

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whats the song?
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Malin Dm's profile photoTimothy O'Flynn's profile photo
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Thanks
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Timothy O'Flynn

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What s the name of the song that plays at 0:42 as he is entering the apartment? 
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In his circles
125 people
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  • University College Cork - National University of Ireland, Cork
    UCC Peer Supporter, 2012 - present
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Ireland, Cork
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Just because I look stupid doesn't mean I'm not.
Education
  • University College Cork - National University of Ireland, Cork
    Genetics, 2011 - present
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Male
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Bollox, Prick, several more..