In a chemical reaction, chemical equilibrium is the state in which both reactants and products are present at concentrations which have no further tendency to change with time. Usually, this state results when the forward reaction proceeds at the same
Different plant parts require different nutrients for their healthy development. So it is important to formulate a medium for plant cell growth which can support the requirements of different organelles of a cell as well as diverse cell types corresponding to each tissue type. Coconut water was used by Van Overbeek in 1941 allowed the culture of young embryos and other recalcitrant tissues including that of monocots, as well as callus cultures of numerous species, including a variety of woody and herbaceous dicots and gymnosperms as well as crown gall tissue cultures were established.