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IJLSSR JOURNAL
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Large Post Auricular Epidermal Inclusion Cyst Involving Facial Nerve- A Rare Case Report

Preeti Singh1*, Vishal Pathania2, Kamal Goyal3

1Assistant Professor, Department of ENT, SGT Medical College and Hospital Gurgaon, India
2Professor, Department of ENT, SGT Medical College and Hospital Gurgaon, India
3Senior Resident, Department of ENT, Medical College and Hospital Gurgaon, India

*Address for Correspondence: Dr. Preeti Singh, Assistant Professor, Department of ENT, SGT Medical College, Farukhnagar Road, Budhera Village, Gurgaon- 122505, India


ABSTRACT
Epidermoid cysts represent most common benign, cutaneous cysts. Most frequently was seen on face, scalp, and trunk. It accounts for approximately 80% of follicular cyst of skin. Epidermoid cyst usually remains asymptomatic until it gets secondarily infected. Malignant changes are seen very rarely. We describe a case of the huge post auricular epidermoid cyst, which was encasing extra-temporal part of the facial nerve and was in close proximity to external carotid artery. The cyst was excised surgically and histopathology confirmed the diagnosis of epidermoid cyst.

Key-words: Epidermoid cyst, Post-auricular cyst, Fine needle aspiration cytology, FNAC
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Analysis of Changing Vegetation Pattern Under Different Climatic, Edaphic and Altitudinal Factors of Doon Valley, Uttarakhand, India

Narendra Kumar1*, Kartik Uniyal2, Zakir Nazir3

1Assistant Professor cum Head, Department of Botany, Alpine Institute of Management & Technology, Dehradun, Uttarakhand, India
2Assistant Professor cum Head, Department of Biotechnology & Microbiology, Alpine Institute of Management & Technology, Dehradun, Uttarakhand, India
3Student, Department of Botany, Alpine Institute of Management & Technology, Dehradun, Uttarakhand, India

*Address for Correspondence: Dr. Narendra Kumar, Assistant Professor cum Head, Department of Botany, Alpine Institute of Management & Technology, Dehradun, Uttarakhand -248 007, India


ABSTRACT
The present study was conducted in altitudinal variable hight and soil pH growing vegetation of Doon valley that the soil type found in Mussoorie is generally medium loamy but its composition, moisture, and pH generally varied from place to place. Higher silt in Mussoorie was due to higher precipitation in the form of winter snow above 1800 m. Soil temperature in Mussoorie ranged from 40C - 160C, while that of Sahastradhara and sudhowala was 15 - 190C. Soil organic matter content tended to be higher in high altitude and increased with increasing altitude. In this study, our finding observed that Soil temperature in Mussoorie ranged from 40C -160C, while that of Sahastradhara was 15 - 190C, Sudhowala 15 - 250C. Soil organic matter content tended to be higher in increase high altitudinal gradient. As per altitudinal variation and soil pH in this region dominant family of angiosperms in Garhwal Himalaya are Asteraceae, Brassicaceae, Cyperaceae, Fabaceae, Lamiaceae, Poaceae, Ranunculaceae, Polygonaceae, Amaranthaceae, Solanaceae, Sexifragaceae, Ranunculaceae Orchidaceae, Apocynaceae, Rutaceae, and Rosaceae.

Key-words: Altitude, Altitudinal Factors Angiosperms, Phytodiversity, Species analysis, Vegetation Pattern, Edaphic
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Microbial Forensics: Forensic Relevance of the Individual Person’s Microbial Signature

Abazuh Uchenna Desmond1*, Oyejide Nicholas2, Olasehinde Olushola Emmanuel3

1Lecturer, Department of Biological sciences, College of Natural Sciences, Redeemer’s University, Ede, Osun, Nigeria
2Laboratory Technologist, Department of Biological sciences, College of Natural Sciences, Redeemer’s University, Ede, Osun, Nigeria
3Research Assistant, Department of Biological sciences, College of Natural Sciences, Redeemer’s University, Ede, Osun, Nigeria

*Address for Correspondence: Dr. Abazuh, Uchenna D, Lecturer, Department of Biological sciences, College of Natural Sciences, Redeemer’s University, Ede, Osun, Nigeria


ABSTRACT
Forensic investigation involves the collecting, assembling, and analysis of all crime-related evidence with the aim of getting to a conclusion about a suspect. Humans have microorganisms present in the gut, mouth, and skin, unique to each individual. Individual microbiome can be distinguished based on the bacterial 16S rRNA to tell the bacterial species diversity between and among persons. Sterilized swab-sticks were used to sample fifteen individuals’ fingertips, their personal items, office doorknob and a college photocopier. Skin-associated bacteria were readily recovered from surfaces and the structure of these bacterial communities can be used to link individuals to the objects they had touched. We compared the bacterial communities on objects and skin to match the objects to the individual. The 16S rRNA gene PCR polymorphism was used to analyze the bacterial community for each person and object. The higher similarity of bacterial community between individuals’ and personal laptop keyboards, office chairs and office member’s fingertips were evident than between the doorknob and the photocopier. Highest bacterial species diversity was observed in doorknob followed by the photocopier. Hence, an individual’s bacterial profile can be used as a human identification tool alongside other tools in forensic fields especially in cases where there is evidence of deficiency.

Key-words: Microbial signature, Forensics, 16S rRNA, Individual person, Skin bacteria, Fingerprint
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Isolation, Characterization, and Application of Calcite Producing Bacteria for Self-Healing Concrete Preparation

Diksha H. Jokyani1, Dharmvir Chouhan2*
1Student, P. G Department of Microbiology, Dhote Bandhu Science College, Gondia, India
2Assistant Professor cum HOD, P. G Department of Microbiology, Dhote Bandhu Science College, Gondia, India
*Address for Correspondence: Prof. D. A. Chouhan, Assistant Professor cum HOD, P.G Department of Microbiology, Dhote Bandhu Science College, Kudwa Road Gondia, Maharashtra- 441614, India


ABSTRACT
Concrete being the foremost building material broadly used in the construction sector is subjected to crack formation due to low tensile strength, durability, and ductility. So this issue is of great curiosity to the researchers in pursuit for the concrete production with better properties. Micro-cracks are the main reason for structural failure occurs, when the load applied exceeds its limits. This causes the seepage of water and other salts. In order to overcome this, the carbonate precipitating, non-pathogenic, spore-forming, alkaline resistant strain of Bacillus subtilis has been explored as a bio-cementing material. Cracks in M20 grade concrete blocks are injected by direct means with screened bacterial strain Bacillus subtilis (BH3) at the cell concentration of 104, 105, 106 CFU/ml with silica gel as an immobilizing agent and calcium lactate as a food source. The cracks were allowed to heal for appropriate time duration at specific pH, temperature, and urea concentration. These findings suggest the potential of Bacillus subtilis in an autogenously healing process.

Key-words: Bacillus subtilis, BH3, Carbonate precipitation, Calcite, Self healing and Bio-cementation
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Phenotypic and Molecular Detection of Mycobacterium avium subsp. paratuberculosis in Small Ruminants Clinically Suspected with Johne᾿s Disease
Mohamed A. I. Hamid1*, Galal Eldin E. Mohammed2, Amel O. Bakhiet3, Elhassan M. A. Saeed4

1Pathologist, Qassim University Teaching Veterinary Hospital, Saudi Arabia
2Professor, Department of Microbiology, Pathology and Parasitology, Faculty of Veterinary Medicine, Sudan University of Science and Technology, Sudan
3Professor, Deanship of Scientific Research, Sudan University of Science and Technology, Sudan
4Associate Professor, Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia

*Address for Correspondence: Mr. Mohamed Alamin Ibrahim Hamid, Pathologist, Qassim University Teaching Veterinary Hospital, Saudi Arabia


ABSTRACT
Johne's disease (JD) or Paratuberculosis (PTB) has gained a great attention by many industrial countries for its severing economic losses and possibly zoonotic concerns. In the current study conventional clinical and direct microscopic examination compared to real time polymerase chain reaction (RT-PCR) were used to diagnose JD in clinically suspected small ruminants. Clinical examination revealed 130 (8.7%) suspected cases that showed history of emaciation and diarrhea out of the total examined (1500) animals. Direct microscopy of Ziehl-Neelsen (ZN) stained smears (130) revealed 62 (47.7%) acid fast bacteria resembled Mycobacterium avium subsp. paratuberculosis (MAP). RT-PCR insertion sequence gene (IS900) detected MAP in 25 (65.8%) out of 38 fecal samples harbored acid fast bacilli. We concluded and recommended that RT-PCR considers the most rapid confirmatory method for screening and diagnosis of the MAP in comparison to low specific conventional phenotypic methods, which still remained valuable techniques in the diagnosis of JD in developing countries.

Key-words: Johne's disease, Paratuberculosis, Acid fast bacteria, Ziehl-Neelsen stain, IS900 gene
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Histopathological and RT-PCR Detection of Mycobacterium paratuberculosis in Tissues of Clinically Suspected Small Ruminants

Mohamed A. I. Hamid1*, Galal Eldin E. Mohammed2, Amel O. Bakhiet3, Elhassan M. A. Saeed4

1Pathologist, Qassim University Teaching Veterinary Hospital, Saudi Arabia
2Professor, Department of Microbiology, Pathology and Parasitology, Faculty of Veterinary Medicine, Sudan University of Science and Technology, Sudan
3Professor, Deanship of Scientific Research, Sudan University of Science and Technology, Sudan
4Associate Professor, Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia

*Address for Correspondence: Mr. Mohamed Alamin Ibrahim Hamid, Pathologist, Qassim University Teaching Veterinary Hospital, Saudi Arabia


ABSTRACT
Paratuberculosis (PTB) remains one of the most obstacles limit animal breeding sector all over the world. The current study aimed to detect the etiology of PTB in tissues of clinically suspected small ruminants using histopathological and real-time polymerase chain reaction (RT-PCR) methods. Clinical examination showed 10 (26.4%) PTB suspected cases out of the total (38) examined animals. The suspected cases were euthanized, necropsied, gross lesions were recorded and tissue samples were collected for histopathological and molecular procedures. Grossly intestinal and mesenteric lymph nodes thickening, corrugations and edematous swellings were recorded. Semi-thin sections of the intestine and mesenteric lymph nodes stained with toluidine blue demonstrated MAP organism inside epithelium cells and macrophages. RT-PCR detected MAP IS900 gene in all suspected cases (100%), thus we recommend using RT-PCR as a rapid sensitive method in the diagnosis of PTB.

Key-words: Paratuberculosis, Mycobacterium, Semi thin sections, Toluidine blue, IS900 gene
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Isolation and Characterization of Pigments from Marine Soil Microorganisms

P. Senthamil Selvi1*, Priya Iyer2

1Research Scholar, Department of Biotechnology, Women’s Christian College, Chennai, India
2Associate Professor, Department of Biotechnology, Women’s Christian College, Chennai, India

*Address for Correspondence: Dr. Priya Iyer, Associate Professor, Department of Biotechnology, Women’s Christian College, Chennai- 600006, India

ABSTRACT
Bacterial pigments have many applications in current day to day life. The pigments produced by chromobacteria can be used for various applications like dairy, pharmaceutical, and food etc. In this study, three types of pigments were isolated i.e. yellow from Xanthomonas sp., pinkish Red from Rhodotorula sp., and orange from Sarcina sp. Pigmented bacterial isolates were obtained from the soil samples and used for the pigment extraction study. We studied that the pigment producing bacteria and identified the color producing pigments. Soil samples from Pondicherry, Cuddalore, Chennai, and Andhra sea coast were collected and used for isolation of microbes producing pigments. Purification of extracted pigments were done by column chromatography, whereas identification and characterization of purified pigment done by UV-Visible spectrophotometry and GC/MS analysis etc. The pigment isolated from bacterial sp. were used for the antimicrobial activity, antioxidant, and anticancer & transformation studies. The bacterial extracts of carotenoid pigment extracted and used as natural colorants for food products and dying of cloth.

Key-words: - Soil samples, GC/MS analysis, UV-Visible spectrophotometry, Carotenoid, Pigment extraction
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Yield of Wheat (Triticum aestivum L.) as Influenced by Planting Date and Planting Methods in the Sudan Savanna Ecological Zone of Nigeria

Na-Allah MS1*, Muhammad A1, Mohammed IU1, Bubuche TS1, Yusif H2, Tanimu MU1

1Department of Crop Science, Faculty of Agriculture, Kebbi State University of Science and Technology, Aliero, Nigeria
2Department of Preliminaries Studies, Waziri Umar Federal Polytechnic Birnin Kebbi, Nigeria

*Address for Correspondence: Mr. Mustapha Sale Na-Allah, Lecturer II, Department of Crop Science Kebbi State University of Science and Technology, Aliero, Nigeria


ABSTRACT
Wheat is a temperate crop requiring low temperatures for growth and yield. Production of wheat in the Sudan savanna agro-ecological zone of Nigeria is restricted to periods of low temperatures that prevailed from early November to late February. Based on the above, field experiments were conducted in two locations at the University Teaching and Research Farm during 2016/2017 dry season to determine the most appropriate planting date and planting method in the study area. The two locations were: Fadama Teaching and Research farm at Jega (Lat. 12°12.99' N; long. 4° 21.90'; 197m above sea level) and the University orchard at Aliero (lat. 12°18.64'N; long. 4°29.85'; 262 above sea level). Both Jega and Aliero are located within Sudan Savanna ecological zone of Nigeria. Treatments consisted of factorial combinations of four Planting dates (1st November, 15th November 1st December and 15th December 2016) and three Planting Methods (Drilling, Dibbling and Broadcasting). The experiments were laid out in a Randomized Complete Block Design (RCBD) with three replications. Results revealed that grain number per spike; grain weight per spike, 1000-grain weight, straw yield, and grain yield were higher when planted on 15th November and 1st December. On the other hand, drilling and dibbling methods resulted to higher stand count, plant height, leave number, leaf area index, days to 50% heading, grain number per spike, grain weight per spike, 1000-grain weight straw and grain yields than broadcasting method. Based on the results of this study, it could be concluded that planting of wheat from 15th November to 1st December coupled with either dibbling or drilling methods of planting gives the best wheat yield in the study area.

Key-words: Ecological Zone, Planting Date, Planting Methods, Savanna, Triticum aestivum
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Expression Profiling of FasL Gene in Human Blood Tissues and its Correlation with Severe Acute Pancreatitis (SAP)

Saurav Biswas1*, Mritunjay Kumar Singh2

1Student, Department of Medical Biotechnology, Sikkim Manipal University, Gangtok, India
2Research Associate, Department of Molecular Biology and Genetics, Allele Life Sciences Pvt. Ltd., Noida, India

*Address for Correspondence: Mr. Saurav Biswas, Student, Department of Medical Biotechnology, Sikkim Manipal University, Gangtok, India


ABSTRACT
Blood leukocytes have a vital role in easing general inflammation through acute pancreatitis. Irrespective of topical growths in the considerate of the intricate pathogenesis of pancreatitis, the existence of the disease remnants sub-optimal. The present work attempts to detect the gene expression level of Fas Ligand (FasL) cDNA in four blood samples. The blood samples consist of different age groups (7 years, 18 years, 25 years, and 50 years), whose expression for the gene FasL was studied by real time PCR. From the study, it was observed, in the sample no (5) of 50 years age the expression is highest followed by sample no (1) of 25 years > sample no (4) of 18 years > sample no (3) of 7 years. The FasL gene is over expressed in the sample no (5) and under expressed in the sample no (1), (4), and (3).

Key-words: FasL, cDNA, Cycle threshold, Apoptosis, Lymphocytes, Severe acute pancreatitis, Immunosuppression
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First Report on Adedes albopictus Subgroup Species in India- Short Communication

Juvin Jose*

Neelankavil House, Kolangattukkara, Choolissery (PO), Thrissur-680 541, Kerala, India

*Address for Correspondence: Mr.Juvin Jose, Research Scholar, Neelankavil (H), Kolangattukkara, Choolissery PO, Thrissur-680541, Kerala, India


ABSTRACT
Adedes albopictus is most advanced stable species trophic level. It is acquired by after great nurture microevolution includes behavioral changes and survivality. In this evolutionary cascade, Ae. albopictus species diverged from its complex species lineage (reported in Kerala Ae. novalbopictus, Ae. pseudalbopictus and Ae. subalbopictus). Without any vestigial the species is pivotal in all the advanced measures include competition, adaptations, reproduction, and intelligence.

Key-words: Adedes albopictus, Subgroup, Microevolution, Evolutionary cascade, Taxonomic study
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